Mouse Anti-FAS Recombinant Antibody (clone DX2) (CAT#: VS3-CJ820)
This product is a mouse antibody that recognizes human FAS (CD95).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/546_F3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/546_F3_1_blue_red_green.jpg
Figure 3 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_9_3.jpg
Figure 4 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_6_6.jpg
Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
Specifications
- Immunogen
- Native purified human CD95
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human FAS (CD95)
- Species Reactivity
- Human
- Clone
- DX2
- Applications
- IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for one year. Do not freeze.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Flow Cytometry.
Target
- Alternative Names
- APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
- Gene ID
- 355
- UniProt ID
- P25445
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions.
Palmitoylated (PubMed:25301068).
Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane (PubMed:25301068).
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Protein Refseq
- NP_000034.1; NP_001307548.1; NP_690610.1
- Function
- Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Related Resources
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Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-094 | Human Anti-FAS Recombinant Antibody (clone HFE7A); scFv Fragment | ELISA, WB, IF, FuncS | Human scFv |
| PSBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); scFv Fragment | WB, IF, FuncS | Human scFv |
| HPAB-0035-YJ-S(P) | Human Anti-FAS Recombinant Antibody (clone h-HFE7A); scFv Fragment | ELISA, Cyt, Apop | Human scFv |
| HPAB-0450-YJ-S(P) | Rabbit Anti-FAS Recombinant Antibody; scFv Fragment (HPAB-0450-YJ-S(P)) | ELISA, FC | Rabbit scFv |
| HPAB-0451-YJ-S(P) | Rabbit Anti-FAS Recombinant Antibody; scFv Fragment (HPAB-0451-YJ-S(P)) | ELISA, FC | Rabbit scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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