Human Anti-MUC1 Recombinant Antibody (clone E29) (CAT#: FAMAB-1222CQ)
This product is a recombinant human antibody that recognizes MUC1. The antibody was purified by affinity chromatography. It can be used in WB, IHC.
Specific Inquiry
IHC
Figure 1 APAAP staining of normal gastric mucosa with antibody E29, showing strong labelling of gastricparietal cells.
In the higher power view the intracanalicular localisation of the antigen is seen (see arrowed cell).
Cordell, J., Richardson, T. C., Pulford, K. A., Ghosh, A. K., Gatter, K. C., Heyderman, E., & Mason, D. Y. (1985). Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. British journal of cancer, 52(3), 347-354.
IHC
Figure 2 APAAP staining of pancreas with antibody E29 shows strong labelling of exocrine glandularelements but no reactivity of islets of Langerhans (IL).
The higher power view shows that labelling is principally confined to the luminal aspect of the glandular cells.
Cordell, J., Richardson, T. C., Pulford, K. A., Ghosh, A. K., Gatter, K. C., Heyderman, E., & Mason, D. Y. (1985). Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. British journal of cancer, 52(3), 347-354.
Figure 3 APAAP staining of kidney with antibody E29 shows strong labelling of distal tubules but noreactivity of proximal tubules or gomeruli.
Cordell, J., Richardson, T. C., Pulford, K. A., Ghosh, A. K., Gatter, K. C., Heyderman, E., & Mason, D. Y. (1985). Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. British journal of cancer, 52(3), 347-354.
Figure 4 APAAP staining of prostate with antibody E29. A strongly labelled glandular structure is seen,with labelling principally on the luminal aspect of the cells. The intensity of the labelling varies markedly inthe prostate, some glandular elements showing no reactivity with antibody E29.
Cordell, J., Richardson, T. C., Pulford, K. A., Ghosh, A. K., Gatter, K. C., Heyderman, E., & Mason, D. Y. (1985). Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. British journal of cancer, 52(3), 347-354.
Figure 5 APAAP staining of normal mesothelium with antibody E29 shows strong staining of the cellsurface.
Cordell, J., Richardson, T. C., Pulford, K. A., Ghosh, A. K., Gatter, K. C., Heyderman, E., & Mason, D. Y. (1985). Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. British journal of cancer, 52(3), 347-354.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Species Reactivity
- Human
- Clone
- E29
- Applications
- WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Mucin 1, Cell Surface Associated; Tumor-Associated Epithelial Membrane Antigen; Breast Carcinoma-Associated Antigen DF3; Peanut-Reactive Urinary Mucin; Polymorphic Epithelial Mucin; Carcinoma-Associated Mucin; Mucin 1, Transmembrane; Krebs Von Den Lungen-6; Cancer Antigen 15-3; Episialin; CA 15-3; H23AG; MUC-1; KL-6; PEMT; EMA; PUM; PEM
- Gene ID
- 4582
- UniProt ID
- P15941
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
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| HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0737-YJ-VHH) | ELISA | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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