Mouse Anti-MUC1 Recombinant Antibody (clone SM3); scFv Fragment

Name: Mouse Anti-MUC1 Recombinant Antibody (clone SM3); scFv Fragment
SKU: PSBC-400
Rating: 5 (1 reviews)

CAT#: PSBC-400

This product is a recombinant mouse antibody scFv fragment that can recognize MUC1. This antibody recognizes the under-glycosylated format of MUC1 and is therefore tumor-specific. The impact of the hydrogen-by-fluorine substitutions on the antigen association to antibody SM3 was evaluated using biolayer interferometry (BLI). The N-acetyl group of the sugar stacks with the aromatic ring of Trp33H provides the driving force for the observed selectivity of SM3 for GalNAc-containing antigens.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg

Normal Tissue Figure 5 Stomach

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg

Normal Tissue Figure 8 Testis

Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg

RNA Expression Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1

❮

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Specifications

Host Species
Mouse

Derivation
Mouse

Type
Mouse scFv

Specificity
Human MUC1

Species Reactivity
Human

Clone
SM3

Applications
FC, IHC-Fr, IF, ELISA, IHC-P

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Gene ID
4582

UniProt ID
P15941

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PSBC-400.pdf

MSDS

MSDS - PSBC-400.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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