Rabbit Anti-MYC Recombinant Antibody (VS3-CJ232)
CAT#: VS3-CJ232
This product is a rabbit antibody that recognizes Phospho-MYC (S62).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human caudate
Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
Normal Tissue
Figure 11 Cervix
Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC
❮
❯
Specifications
- Immunogen
- KLH-conjugated synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MYC
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39
- Gene ID
- 4609
- UniProt ID
- P01106
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by PRKDC (PubMed:1597196).
Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity).
Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526).
Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58 (PubMed:22307329).
Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367).
Dephosphorylation at Ser-62 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25803737, PubMed:25438055).
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:25775507).
In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation (PubMed:17873522, PubMed:17558397).
In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus (PubMed:17558397, PubMed:25775507).
Also polyubiquitinated by the DCX(TRPC4AP) complex (PubMed:20551172, PubMed:29779948).
Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).
- Protein Refseq
- NP_002458.2
- Function
- Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029).
Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029).
Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029).
Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity).
Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).
Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
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Gastric Cancer
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MYC"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-281 | Mouse Anti-MYC Recombinant Antibody (clone 9E10); scFv Fragment | WB, IHC, FuncS | Mouse scFv |
| FAMAB-0112-CN-S(P) | Mouse Anti-MYC Recombinant Antibody (clone CT14); scFv Fragment | ELISA, WB, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-281 | Mouse Anti-MYC Recombinant Antibody (clone 9E10); Fab Fragment | WB, IHC, FuncS | Mouse Fab |
| FAMAB-0112-CN-F(E) | Mouse Anti-MYC Recombinant Antibody (clone CT14); Fab Fragment | ELISA, WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0400MC | Anti-c-Myc antibody (HRP) | ELISA, IHC-P, IHC-Fr, WB |
| CAT | Product Name | Application | Type |
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| BRD-0380MZ | Chicken Anti-c-Myc (ab1) Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2336 | Hi-Affi™ Recombinant Rabbit Anti-MYC Monoclonal Antibody (DS2336AB) | WB, IP, ICC | IgG |
| MOR-4554 | Hi-Affi™ Recombinant Rabbit Anti-MYC Monoclonal Antibody (TH64DS) | WB, IF, ICC, FC | IgG |
| MOR-0050-FY | Rabbit Anti-MYC Recombinant Antibody (clone AFY0021) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2311-CN | Recombinant Rabbit Anti-MYC (phosphorylated Thr58) Monoclonal Antibody (CBACN-604) | WB, IF, FC | Rabbit IgG |
| MRO-2312-CN | Recombinant Rabbit Anti-MYC (phosphorylated Thr58+Ser62) Monoclonal Antibody (CBACN-605) | WB, IF, IP, FC | Rabbit IgG |
| MRO-2313-CN | Recombinant Rabbit Anti-MYC (phosphorylated Ser62) Monoclonal Antibody (CBACN-606) | WB, IF, IHC, IP | Rabbit IgG |
| VS-0223-WK12 | Rabbit Anti-MYC Recombinant Antibody (VS-0223-WK12) | WB, IP, ICC, IHC, IHC-P | Rabbit IgG |
| VS3-XY3440 | Rabbit Anti-MYC Recombinant Antibody (clone R03-1I8) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY194 | AbPlus™ Anti-MYC Magnetic Beads (33) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY133 | Mouse Anti-NHP MYC Recombinant Antibody (clone MYC699) | IF, FC, ELISA | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4607 | Anti-MYC Immunohistochemistry Kit | IHC | |
| VS-0525-XY4608 | Anti-Human MYC Immunohistochemistry Kit | IHC | |
| VS-0525-XY4609 | Anti-Mouse MYC Immunohistochemistry Kit | IHC |
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