Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0080-LSX-F(E)) (CAT#: HPAB-0080-LSX-F(E))
HPAB-0080-LSX binds to the Ig-like V domain of huCD33 and is rapidly internalized by HL-60 cells within 30 minutes. The localization of HPAB-0080-LSX to the lysosome was confirmed using an in vitro cell-based assay that measures the ability of an antibody drug conjugate to kill target cells. HPAB-0080-LSX-F(E) antibody is the Fab fragment antibody form of HPAB-0080-LSX antibody. Fab fragments, the antigen-binding domains of an antibody molecule, consist of two amino acid chains composed of VH+CH1 and VL+CL domains.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Figure 4 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Figure 6 Kidney
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Figure 8 Spleen
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
Specifications
- Immunogen
- Recombinant hu and cyno CD33 extracellular domain
- Host Species
- Human
- Derivation
- Human
- Type
- Human Fab
- Specificity
- Human CD33
- Species Reactivity
- Human, Cynomolgus monkey
- Applications
- ELISA
- Related Disease
- Human Acute Myeloid Leukemia
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD33 Molecule; Sialic Acid Binding Ig-Like Lectin 3; Sialic Acid-Binding Ig-Like Lectin 3; CD33 Antigen (Gp67); SIGLEC-3; SIGLEC3; Gp67; CD33 Antigen; P67
- Gene ID
- 945
- UniProt ID
- P20138
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-321-sdAb | Recombinant Anti-human CD33 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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