Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0080-LSX-F(E))

Name: Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0080-LSX-F(E))
SKU: HPAB-0080-LSX-F(E)
Rating: 5 (1 reviews)

CAT#: HPAB-0080-LSX-F(E)

HPAB-0080-LSX binds to the Ig-like V domain of huCD33 and is rapidly internalized by HL-60 cells within 30 minutes. The localization of HPAB-0080-LSX to the lysosome was confirmed using an in vitro cell-based assay that measures the ability of an antibody drug conjugate to kill target cells. HPAB-0080-LSX-F(E) antibody is the Fab fragment antibody form of HPAB-0080-LSX antibody. Fab fragments, the antigen-binding domains of an antibody molecule, consist of two amino acid chains composed of VH+CH1 and VL+CL domains.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line PC-3

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg

Normal Tissue Figure 6 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg

Normal Tissue Figure 8 Spleen

Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

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Specifications

Immunogen
Recombinant hu and cyno CD33 extracellular domain

Host Species
Human

Derivation
Human

Type
Human Fab

Specificity
Human CD33

Species Reactivity
Human, Cynomolgus monkey

Applications
ELISA

Related Disease
Human Acute Myeloid Leukemia

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CD33 Molecule; Sialic Acid Binding Ig-Like Lectin 3; Sialic Acid-Binding Ig-Like Lectin 3; CD33 Antigen (Gp67); SIGLEC-3; SIGLEC3; Gp67; CD33 Antigen; P67

Gene ID
945

UniProt ID
P20138

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0080-LSX-F(E).pdf

MSDS

MSDS - HPAB-0080-LSX-F(E).pdf

COA

Certificate of Analysis Lookup

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