Anti-CD33 Recombinant Antibody (TAB-756) (CAT#: TAB-756)

Recombinant humanized (from mouse) antibody expressed in CHO binding to human CD33. It is a humanized monoclonal antibody used in the treatment of cancer.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Isotype Switching:
  • Fc Engineering:
  • Modalities:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Anti-Human CD33 Antibody in WB

Figure 1 Anti-Human CD33 Antibody in WB

Western blot analysis of TAB-756 was performed by loading Recombinant Human CD33 Protein onto a 12% Tris-HCl polyacrylamide gel. The secondary antibody: Goat Anti-Human IgG-HRP.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)

ELISA

Figure 2 Anti-Human CD33 Antibody in ELISA

Figure 2 Anti-Human CD33 Antibody in ELISA

ELISA analysis of TAB-756 was performed by coating with Recombinant Human CD33 Protein. Then blocked with BSA, and incubated with Anti-Human CD33 Antibody (TAB-756). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

SDS-PAGE

Figure 3 Anti-CD33 Recombinant Antibody (TAB-756) in SDS-PAGE

Figure 3 Anti-CD33 Recombinant Antibody (TAB-756) in SDS-PAGE

SDS-PAGE analysis of TAB-756 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.

SEC-HPLC

Figure 4 Anti-CD33 Recombinant Antibody (TAB-756) in SEC-HPLC

Figure 4 Anti-CD33 Recombinant Antibody (TAB-756) in SEC-HPLC

HPLC analysis of TAB-756 shows that the purity is more than 95%.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG1 - kappa
  • Species Reactivity
  • Human
  • Applications
  • IF, IP, Neut, FuncS, ELISA, FC, ICC
  • Related Disease
  • Acute myeloid leukemia (AML)

Product Property

  • Purity
  • >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The CD33 antibody has been reported in applications of Activ, FC, Stim.

Target

  • Alternative Names
  • lintuzumab;zamyl/smart;166089-32-3;SGN-33;HuM195;Actimab-A;Lintuzumab-Ac-225;SGN-33;CD33;CD33 molecule;CD33 antigen (gp67);myeloid cell surface antigen CD33;FLJ00391;p67;sialic acid binding Ig like lectin 3;SIGLEC 3;SIGLEC3;gp67;sialic acid binding Ig-lik

Related Resources

  • Biosimilar Overview
  • Citations
Please refer to Lintuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Lintuzumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD33"

Select a product category from the dropdown menu below to view related products.
Please select product type
Single Domain Antibody Products Humanized Antibody Products Immunotoxin Products Mouse Antibody Products Human Antibody Products Fab Glycosylation Antibody Products MHC Tetramer Products for Cancer Blocking Antibody Products Rabbit Monoclonal Antibody Products Chimeric Antibody Products ScFv Antibody Products ADCC Enhanced Antibody Products Fab Antibody Products IgG Antibody Products Cancer Stem Cell Biomarker Specific Antibody Products
CAT Product Name Application Type
NAB-321-sdAb Recombinant Anti-human CD33 VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

Customer Reviews and Q&As

Customer Review Q&As

Submit a review or a question

  • Ideal for CD33 Research
    The Anti-CD33 Therapeutic Antibody (ZAMYL) has been excellent for our CD33 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
  • Reliable in Immunoassays
    We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CD33 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
  • Consistent and High-Quality
    This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for CD33 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-756, RRID: AB_3112003)

Copy citation

Popular products with customers


For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

  • 0
  • 0
Cart
    Go to compare

    Go to compare