Anti-CD33 Recombinant Antibody (TAB-756) (CAT#: TAB-756)
Recombinant humanized (from mouse) antibody expressed in CHO binding to human CD33. It is a humanized monoclonal antibody used in the treatment of cancer.
Specific Inquiry
WB
Figure 1 Anti-Human CD33 Antibody in WB
Western blot analysis of TAB-756 was performed by loading Recombinant Human CD33 Protein onto a 12% Tris-HCl polyacrylamide gel. The secondary antibody: Goat Anti-Human IgG-HRP.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)
ELISA
Figure 2 Anti-Human CD33 Antibody in ELISA
ELISA analysis of TAB-756 was performed by coating with Recombinant Human CD33 Protein. Then blocked with BSA, and incubated with Anti-Human CD33 Antibody (TAB-756). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
Figure 3 Anti-CD33 Recombinant Antibody (TAB-756) in SDS-PAGE
SDS-PAGE analysis of TAB-756 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.
SEC-HPLC
Figure 4 Anti-CD33 Recombinant Antibody (TAB-756) in SEC-HPLC
HPLC analysis of TAB-756 shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Species Reactivity
- Human
- Applications
- IF, IP, Neut, FuncS, ELISA, FC, ICC
- Related Disease
- Acute myeloid leukemia (AML)
Product Property
- Purity
- >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The CD33 antibody has been reported in applications of Activ, FC, Stim.
Target
- Alternative Names
- lintuzumab;zamyl/smart;166089-32-3;SGN-33;HuM195;Actimab-A;Lintuzumab-Ac-225;SGN-33;CD33;CD33 molecule;CD33 antigen (gp67);myeloid cell surface antigen CD33;FLJ00391;p67;sialic acid binding Ig like lectin 3;SIGLEC 3;SIGLEC3;gp67;sialic acid binding Ig-lik
- Gene ID
- 945
- UniProt ID
- P20138
Related Resources
Please refer to Lintuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Lintuzumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-321-sdAb | Recombinant Anti-human CD33 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
Customer Reviews and Q&As
Customer Review
Q&As
-
Ideal for CD33 Research
The Anti-CD33 Therapeutic Antibody (ZAMYL) has been excellent for our CD33 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time. -
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CD33 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab. -
Consistent and High-Quality
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for CD33 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-756, RRID: AB_3112003)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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