Recombinant Anti-human CD33 VHH Single Domain Antibody
CAT#: NAB-321-sdAb
Anti-human CD33 VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Normal Tissue
Figure 8 Spleen
Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
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Specifications
- Immunogen
- human CD33 molecule
- Host Species
- Llama
- Type
- Llama VHH
- Species Reactivity
- Human
- Molecule Class
- VHH Single Domain Antibody
- Applications
- This product is suitable for use in WB, IP, ChiP, Neut, ELISA and most other immunological methods.
Product Property
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
REVIEWS AND Q&AS
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Promising results
The results with the Recombinant Anti-human CD33 VHH Single Domain Antibody have been very promising. The leukemic cell counts have significantly decreased, and the side effects are minimal.
Excellent for ELISA
I've been using the antibody in my laboratory for ELISA tests, and it's been a game-changer. The binding affinity and specificity for human CD33 molecule are excellent.
Emily Parker09.Apr,22
High specificity and low background for IA
The recombinant Anti-human CD33 VHH Single Domain Antibody exceeded our expectations. The high specificity and low background have significantly improved the quality of our immunoassays.
Q&As
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What is the Recombinant Anti-human CD33 VHH Single Domain Antibody's major application?
A: The primary use of the Recombinant Anti-human CD33 VHH Single Domain Antibody is to target and treat acute myeloid leukemia (AML) by binding specifically to the CD33 antigen on leukemic cells, facilitating their identification and destruction by the immune system, while sparing healthy cells.
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Does the Recombinant Anti-human CD33 VHH Single Domain Antibody have any contraindications when used?
A: Contraindications may include individuals with known hypersensitivity to any components of the antibody formulation. It should also be used with caution in patients with severe pre-existing conditions that could be exacerbated by the treatment. Consulting a healthcare provider is essential to assess individual suitability.
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How is harm to healthy cells reduced by the Recombinant Anti-human CD33 VHH Single Domain Antibody?
A: The antibody's small size and specificity for the CD33 antigen allow it to bind only to leukemic cells, sparing most healthy cells. This specificity reduces the broad, systemic toxicity commonly associated with traditional treatments, resulting in fewer and less severe side effects.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CD33"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-013 | Humanized Anti-CD33 Recombinant Antibody (TAB-013) | IF, IP, Neut, FuncS, ELISA, FC, WB | Humanized (from mouse) IgG4, κ |
| TAB-H37 | Anti-Human CD33 Recombinant Antibody (Gemtuzumab Ozogamicin) | ELISA, FC, IP, FuncS, IF, Neut, IHC | IgG |
| TAB-756 | Anti-CD33 Recombinant Antibody (TAB-756) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| VS3-WK1523 | Human Anti-CD33 Recombinant Antibody (clone CDP-771) | FC | Humanized IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G008E | Anti-CD33 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G008G | Anti-CD33 immunotoxin (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-L050E | anti-CD33 immunotoxin M195 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L050G | anti-CD33 immunotoxin M195 (IgG)-Gel | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-119LC | Mouse Anti-CD33 Recombinant Antibody (TAB-119LC) | ELISA, IF, RIA | Mouse IgG |
| TAB-120LC | Mouse Anti-CD33 Recombinant Antibody (TAB-120LC) | ELISA, Cyt | Mouse IgG |
| TAB-121LC | Mouse Anti-CD33 Recombinant Antibody (TAB-121LC) | ELISA | Mouse IgG |
| TAB-122LC | Mouse Anti-CD33 Recombinant Antibody (TAB-122LC) | ELISA, Cyt | Mouse IgG |
| TAB-124LC | Mouse Anti-CD33 Recombinant Antibody (TAB-124LC) | ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-123LC | Anti-Human CD33 Recombinant Antibody | FC | Human antibody |
| TAB-146LC | Human Anti-CD33 Recombinant Antibody (TAB-146LC) | ELISA | Human IgG |
| TAB-123LC-S(P) | Anti-Human CD33 Recombinant Antibody scFv Fragment | FC | Human antibody |
| TAB-146LC-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) | ELISA | Human scFv |
| TAB-123LC-F(E) | Anti-Human CD33 Recombinant Antibody Fab Fragment | FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-013LC | Recombinant Anti-Human CD33 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-341CQ | Mouse Anti-CD33 Recombinant Antibody (clone WM53) | FC, IHC-Fr, ICC, IP, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0553 | Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-471CQ | Anti-Human CD33 Recombinant Antibody (TAB-471CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0045CQ-S(P) | Human Anti-CD33 Recombinant Antibody (clone huM195); scFv Fragment | ELISA, FC | Humanized scFv |
| HPAB-0307-FY-S(P) | Human Anti-CD33 Recombinant Antibody scFv Fragment (HPAB-0307-FY-S(P)) | WB | Humanized scFv |
| HPAB-1910LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1910LY-S(P)) | FC | Humanized scfv |
| HPAB-1911LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1911LY-S(P)) | FC | Humanized scfv |
| HPAB-1912LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1912LY-S(P)) | FC | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0087-FY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0087-FY-F(E)) | ELISA | Human Fab |
| HPAB-0307-FY-F(E) | Human Anti-CD33 Recombinant Antibody Fab Fragment (HPAB-0307-FY-F(E)) | WB | Humanized Fab |
| HPAB-1981LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1981LY-F(E)) | FC | Humanized Fab |
| HPAB-1982LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1982LY-F(E)) | FC | Humanized Fab |
| HPAB-1983LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1983LY-F(E)) | FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-471CQ | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-471CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-756 | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-756) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-013 | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-013) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1910LY | Human Anti-CD33 Recombinant Antibody (HPAB-1910LY) | FC, Inhib, FuncS | Humanized IgG |
| HPAB-1911LY | Human Anti-CD33 Recombinant Antibody (HPAB-1911LY) | FC, SPR, FuncS | Humanized IgG |
| HPAB-1912LY | Human Anti-CD33 Recombinant Antibody (HPAB-1912LY) | FC | Humanized IgG |
| HPAB-1913LY | Human Anti-CD33 Recombinant Antibody (HPAB-1913LY) | FC, SPR | Humanized IgG |
| HPAB-1914LY | Human Anti-CD33 Recombinant Antibody (HPAB-1914LY) | FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0509 | PE-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-CN0510 | APC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-CN0511 | FITC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-CN0512 | A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-YF358 | A*02:01/Human CD33 65-73 (1Y2L) (YLISGDSPV) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC24 | Rabbit Anti-CD33 Antibody (VS-0924-YC24) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY35 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone HIM3-4) | FC | Mouse IgG1, kappa |
| VS-0225-XY36 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone P67.6) | FC | Mouse IgG1, kappa |
| VS-0225-XY37 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone WM53) | FC | Mouse IgG1, kappa |
| VS13-YC160 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (VS13-YC160) | IHC-P, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY84 | Mouse Anti-CD33 scFv-Fc Chimera (VS-0425-FY84) | FC, IF | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC365 | Recombinant Anti-CD33 Vesicular Antibody, EV Displayed (VS-0425-YC365) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1159 | Anti-CD33 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1160 | Anti-Human CD33 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC28 | Recombinant Anti-CD33 (IgV domain x IgC domain) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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