Does the Recombinant Anti-human CD33 VHH Single Domain Antibody have any contraindications when used?

Contraindications may include individuals with known hypersensitivity to any components of the antibody formulation. It should also be used with caution in patients with severe pre-existing conditions that could be exacerbated by the treatment. Consulting a healthcare provider is essential to assess individual suitability.

How is harm to healthy cells reduced by the Recombinant Anti-human CD33 VHH Single Domain Antibody?

The antibody's small size and specificity for the CD33 antigen allow it to bind only to leukemic cells, sparing most healthy cells. This specificity reduces the broad, systemic toxicity commonly associated with traditional treatments, resulting in fewer and less severe side effects.

Recombinant Anti-human CD33 VHH Single Domain Antibody

CAT#: NAB-321-sdAb

Anti-human CD33 VHH Single Domain Antibody is a recombinant protein produced in E. coli.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line PC-3

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg

Normal Tissue Figure 6 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg

Normal Tissue Figure 8 Spleen

Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

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Specifications

Immunogen
human CD33 molecule

Host Species
Llama

Type
Llama VHH

Species Reactivity
Human

Molecule Class
VHH Single Domain Antibody

Applications
This product is suitable for use in WB, IP, ChiP, Neut, ELISA and most other immunological methods.

Product Property

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

Alternative Names
CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3

Gene ID
945

UniProt ID
P20138

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Customer Review

There are currently no Customer reviews or questions for NAB-321-sdAb. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Promising results

The results with the Recombinant Anti-human CD33 VHH Single Domain Antibody have been very promising. The leukemic cell counts have significantly decreased, and the side effects are minimal.

Excellent for ELISA

I've been using the antibody in my laboratory for ELISA tests, and it's been a game-changer. The binding affinity and specificity for human CD33 molecule are excellent.

Emily Parker

High specificity and low background for IA

The recombinant Anti-human CD33 VHH Single Domain Antibody exceeded our expectations. The high specificity and low background have significantly improved the quality of our immunoassays.

Q&As

What is the Recombinant Anti-human CD33 VHH Single Domain Antibody's major application?

A: The primary use of the Recombinant Anti-human CD33 VHH Single Domain Antibody is to target and treat acute myeloid leukemia (AML) by binding specifically to the CD33 antigen on leukemic cells, facilitating their identification and destruction by the immune system, while sparing healthy cells.
Does the Recombinant Anti-human CD33 VHH Single Domain Antibody have any contraindications when used?

A: Contraindications may include individuals with known hypersensitivity to any components of the antibody formulation. It should also be used with caution in patients with severe pre-existing conditions that could be exacerbated by the treatment. Consulting a healthcare provider is essential to assess individual suitability.
How is harm to healthy cells reduced by the Recombinant Anti-human CD33 VHH Single Domain Antibody?

A: The antibody's small size and specificity for the CD33 antigen allow it to bind only to leukemic cells, sparing most healthy cells. This specificity reduces the broad, systemic toxicity commonly associated with traditional treatments, resulting in fewer and less severe side effects.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NAB-321-sdAb.pdf

MSDS

MSDS - NAB-321-sdAb.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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