Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-013)
CAT#: AFC-TAB-013
This product is an ADCC enhanced antibody produced by our Afuco™ platform. It is a monoclonal antibody to CD33.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Normal Tissue
Figure 8 Spleen
Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Applications
- IF, IP, Neut, FuncS, ELISA, FC
- Conjugate
- Unconjugated
- Trade name
- Mylotarg
- Generic Name
- Gemtuzumab
Product Property
- Purity
- >95% by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
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Excellent for Targeting Cancer Cells
Gemtuzumab has been a game-changer in our lab for targeting CD33-positive cancer cells. Its ability to enhance ADCC helps us study immune responses more effectively, paving the way for potential new therapies.
Perfect for Flow Cytometry
We've been using Gemtuzumab in flow cytometry, and the results are consistently precise and clear. It's indispensable for identifying and studying CD33-positive cell populations in our experiments.
Advanced Metabolic Studies
Gemtuzumab has facilitated advanced studies on cell metabolism during activation by engaging CD33. Understanding these metabolic pathways is crucial for revealing critical aspects of T-cell function.
Q&As
-
How does gemtuzumab, also known as AfucoTM Anti-CD33 ADCC Recombinant Antibody, target cancer cells?
A: Gemtuzumab targets CD33, a protein commonly found on the surface of certain cancer cells, especially in acute myeloid leukemia (AML). It enhances Antibody-Dependent Cellular Cytotoxicity (ADCC), meaning it helps the immune system's natural killer cells to better identify and destroy these cancer cells.
-
In what ways does gemtuzumab improve research on immune responses directed against cancer cells?
A: By targeting CD33 and enhancing ADCC, Gemtuzumab allows researchers to study how effectively the immune system's natural killer cells can attack and kill cancer cells, giving a clearer picture of potential immune responses in AML and other CD33-positive cancers.
-
When compared to other antibodies, what are the advantages of utilizing gemtuzumab in cancer research?
A: Gemtuzumab is specifically engineered to enhance ADCC, making it more effective in promoting immune-mediated killing of CD33-positive cancer cells. This enhanced activity provides more robust experimental outcomes and greater potential for therapeutic applications.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| TAB-119LC | Mouse Anti-CD33 Recombinant Antibody (TAB-119LC) | ELISA, IF, RIA | Mouse IgG |
| TAB-120LC | Mouse Anti-CD33 Recombinant Antibody (TAB-120LC) | ELISA, Cyt | Mouse IgG |
| TAB-121LC | Mouse Anti-CD33 Recombinant Antibody (TAB-121LC) | ELISA | Mouse IgG |
| TAB-122LC | Mouse Anti-CD33 Recombinant Antibody (TAB-122LC) | ELISA, Cyt | Mouse IgG |
| TAB-124LC | Mouse Anti-CD33 Recombinant Antibody (TAB-124LC) | ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-123LC | Anti-Human CD33 Recombinant Antibody | FC | Human antibody |
| TAB-146LC | Human Anti-CD33 Recombinant Antibody (TAB-146LC) | ELISA | Human IgG |
| TAB-123LC-S(P) | Anti-Human CD33 Recombinant Antibody scFv Fragment | FC | Human antibody |
| TAB-146LC-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) | ELISA | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-013LC | Recombinant Anti-Human CD33 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC162 | APC-A*02:01/Human CD33 (AIISGDSPV) MHC Tetramer | FCM | |
| MHC-CN0509 | PE-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-CN0510 | APC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-CN0511 | FITC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC | |
| MHC-CN0512 | A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0553 | Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-471CQ | Anti-Human CD33 Recombinant Antibody (TAB-471CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0077-LSX-S(P) | Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-0077-LSX-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0078-LSX-S(P) | Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-0078-LSX-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0079-LSX-S(P) | Mouse Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-0079-LSX-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0080-LSX-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-0080-LSX-S(P)) | ELISA | Human scFv |
| HPAB-0081-LSX-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-0081-LSX-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0077-LSX-F(E) | Mouse Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0077-LSX-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0078-LSX-F(E) | Mouse Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0078-LSX-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0079-LSX-F(E) | Mouse Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0079-LSX-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0080-LSX-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0080-LSX-F(E)) | ELISA | Human Fab |
| HPAB-0081-LSX-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0081-LSX-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-471CQ | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-471CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-756 | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-756) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1910LY | Human Anti-CD33 Recombinant Antibody (HPAB-1910LY) | FC, Inhib, FuncS | Humanized IgG |
| HPAB-1911LY | Human Anti-CD33 Recombinant Antibody (HPAB-1911LY) | FC, SPR, FuncS | Humanized IgG |
| HPAB-1912LY | Human Anti-CD33 Recombinant Antibody (HPAB-1912LY) | FC | Humanized IgG |
| HPAB-1913LY | Human Anti-CD33 Recombinant Antibody (HPAB-1913LY) | FC, SPR | Humanized IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC24 | Rabbit Anti-CD33 Antibody (VS-0924-YC24) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0225-XY36 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone P67.6) | FC | Mouse IgG1, kappa |
| VS-0225-XY37 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone WM53) | FC | Mouse IgG1, kappa |
| VS13-YC160 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (VS13-YC160) | IHC-P, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0425-FY84 | Mouse Anti-CD33 scFv-Fc Chimera (VS-0425-FY84) | FC, IF | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY1159 | Anti-CD33 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1160 | Anti-Human CD33 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-YC28 | Recombinant Anti-CD33 (IgV domain x IgC domain) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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