CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone P67.6) (CAT#: VS-0225-XY36)
This mouse-derived antibody targets CD33, a key marker for hematopoietic and immune cells. With strong reactivity to human samples, it is optimized for flow cytometry applications, providing precise detection and analysis of CD33-expressing cells. Ideal for research into hematological diseases and immune responses, this antibody ensures reliable performance in flow cytometry assays, offering researchers a powerful tool for cellular profiling and immune monitoring.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Figure 4 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Figure 6 Kidney
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Figure 8 Spleen
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
Specifications
- Immunogen
- CD33-transfected FMY9S5 Cells
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CD33
- Species Reactivity
- Human
- Clone
- P67.6
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
- Alternative Names
- Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9
- Gene ID
- 945
- UniProt ID
- P20138
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-321-sdAb | Recombinant Anti-human CD33 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
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