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  • Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB)

Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) (CAT#: MOR-0553)

Recombinant Rabbit anti-CD33 monoclonal antibody for WB, ICC, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.


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  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Figure 2 IHC staining of human lymph node
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Figure 4 IF staining of human cell line RH-30
Figure 4 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Figure 8 Spleen
Figure 8 Spleen
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

Specifications

  • Host Species
  • Rabbit
  • Derivation
  • Phage display library screening
  • Type
  • Rabbit IgG
  • Species Reactivity
  • Human
  • Clone
  • DS553AB
  • Applications
  • WB, ICC, IP
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Tissue culture supernatant
  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Format
  • Liquid
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • CD33 Molecule; Sialic Acid Binding Ig-Like Lectin 3; Sialic Acid-Binding Ig-Like Lectin 3; CD33 Antigen (Gp67); SIGLEC-3; SIGLEC3; Gp67; CD33 Antigen; P67

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD33"

Single-domain Antibody

CAT Product Name Application Type
NAB-321-sdAb Recombinant Anti-human CD33 VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-013 Humanized Anti-CD33 Recombinant Antibody (clone Gemtuzumab) IF, IP, Neut, FuncS, ELISA, FC, WB Humanized (from mouse) IgG4, κ
TAB-H37 Anti-Human CD33 Recombinant Antibody (Gemtuzumab Ozogamicin) ELISA, FC, IP, FuncS, IF, Neut, IHC IgG
TAB-756 Anti-CD33 Recombinant Antibody (Lintuzumab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
VS3-WK1523 Human Anti-CD33 Recombinant Antibody (clone CDP-771) FC Humanized IgG4

Immunotoxin

CAT Product Name Application Type
AGTO-G008E Anti-CD33 immunotoxin (scFv)-PE Cytotoxicity assay, Function study
AGTO-G008G Anti-CD33 immunotoxin (scFv)-Gel Cytotoxicity assay, Function study
AGTO-L050E anti-CD33 immunotoxin M195 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L050G anti-CD33 immunotoxin M195 (IgG)-Gel Cytotoxicity assay, Functional assay

Mouse Antibody

CAT Product Name Application Type
TAB-119LC Mouse Anti-CD33 Recombinant Antibody (TAB-119LC) ELISA, IF, RIA Mouse IgG
TAB-120LC Mouse Anti-CD33 Recombinant Antibody (TAB-120LC) ELISA, Cyt Mouse IgG
TAB-121LC Mouse Anti-CD33 Recombinant Antibody (TAB-121LC) ELISA Mouse IgG
TAB-122LC Mouse Anti-CD33 Recombinant Antibody (TAB-122LC) ELISA, Cyt Mouse IgG
TAB-124LC Mouse Anti-CD33 Recombinant Antibody (TAB-124LC) ELISA, FC Mouse IgG

Human Antibody

CAT Product Name Application Type
TAB-123LC Anti-Human CD33 Recombinant Antibody FC Human antibody
TAB-146LC Human Anti-CD33 Recombinant Antibody (TAB-146LC) ELISA Human IgG
TAB-123LC-S(P) Anti-Human CD33 Recombinant Antibody scFv Fragment FC Human antibody
TAB-146LC-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) ELISA Human scFv
TAB-123LC-F(E) Anti-Human CD33 Recombinant Antibody Fab Fragment FC Human antibody

Fab Glycosylation

CAT Product Name Application Type
Gly-013LC Recombinant Anti-Human CD33 Antibody (Fab glycosylation) ELISA Humanized antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC162 APC-A*02:01/Human CD33 (AIISGDSPV) MHC Tetramer FCM
MHC-LC163 PE-A*02:01/Human CD33 A65Y (YIISGDSPV) MHC Tetramer FCM
MHC-LC164 APC-A*02:01/Human CD33 A65Y (YIISGDSPV) MHC Tetramer FCM
MHC-LC2064 PE-A*02:01/Human CD33 (AIISGDSPV) MHC Tetramer FCM
MHC-YF358 A*02:01/Human CD33 65-73 (1Y2L) (YLISGDSPV) MHC Pentamer FCM

Blocking Antibody

CAT Product Name Application Type
NEUT-341CQ Mouse Anti-CD33 Recombinant Antibody (clone WM53) FC, IHC-Fr, ICC, IP, WB, Block Mouse IgG1, κ

Chimeric Antibody

CAT Product Name Application Type
TAB-471CQ Anti-Human CD33 Recombinant Antibody (Vadastuximab) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

Recombinant Antibody

CAT Product Name Application Type
HPAB-0077-LSX Mouse Anti-CD33 Recombinant Antibody (HPAB-0077-LSX) ELISA, FC, SPR Mouse IgG
HPAB-0078-LSX Mouse Anti-CD33 Recombinant Antibody (HPAB-0078-LSX) ELISA, FC, SPR Mouse IgG
HPAB-0079-LSX Mouse Anti-CD33 Recombinant Antibody (HPAB-0079-LSX) ELISA, FC, SPR Mouse IgG
HPAB-0080-LSX Human Anti-CD33 Recombinant Antibody (HPAB-0080-LSX) ELISA, FC Human IgG
HPAB-0081-LSX Human Anti-CD33 Recombinant Antibody (HPAB-0081-LSX) ELISA, FC Human IgG

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0045CQ-S(P) Human Anti-CD33 Recombinant Antibody (clone huM195); scFv Fragment ELISA, FC Humanized scFv
HPAB-1914LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1914LY-S(P)) FC Humanized scfv
HPAB-1917LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1917LY-S(P)) FC Humanized scfv
HPAB-1918LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1918LY-S(P)) FC Humanized scfv
HPAB-1919LY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1919LY-S(P)) FC Humanized scfv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0307-FY-F(E) Human Anti-CD33 Recombinant Antibody (clone GO); Fab Fragment WB Humanized Fab
HPAB-1910LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1910LY-F(E)) FC Humanized Fab
HPAB-1911LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1911LY-F(E)) FC Humanized Fab
HPAB-1912LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1912LY-F(E)) FC Humanized Fab
HPAB-1913LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1913LY-F(E)) FC Humanized Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-471CQ Afuco™ Anti-CD33 ADCC Recombinant Antibody (Vadastuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-756 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Lintuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-013 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Gemtuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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