Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-756)
CAT#: AFC-TAB-756
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant humanized (from mouse) antibody expressed in CHO binding to human CD33. It is a humanized monoclonal antibody used in the treatment of cancer.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Normal Tissue
Figure 8 Spleen
Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Applications
- IF, IP, Neut, FuncS, ELISA, FC
- Conjugate
- Unconjugated
- Trade name
- Zamyl/Smart
- Generic Name
- Lintuzumab
- Related Disease
- Acute myeloid leukemia (AML)
Product Property
- Purity
- >95% by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Customer Review
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Excellent for Studying Cancer Cells
Lintuzumab has been transformative for our research on CD33-positive cancer cells. Its enhanced ADCC capability has allowed us to study immune responses more effectively, laying the groundwork for potential new therapies.
High Specificity
Lintuzumab consistently demonstrates high specificity for CD33, with minimal off-target effects. This specificity ensures our results are accurate, making it indispensable for our CD33-positive cancer research.
Robust Functional Analysis
The data we've gained from using Lintuzumab in functional assays has been robust and reliable. It has played a crucial role in understanding ADCC dynamics and the therapeutic targeting of CD33-positive cells.
Q&As
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How should lintuzumab be stored to maintain its effectiveness?
A: To ensure Lintuzumab remains effective, store it at -20°C or lower. Aliquot it upon first use to avoid repeated thawing and refreezing, which can degrade its efficacy over time.
-
How can I figure out how much lintuzumab is best for my experiments?
A: Perform a titration by testing a range of concentrations, typically from 0.1 μg/ml to 10 μg/ml. This helps identify the concentration that provides effective binding and ADCC activity in your specific experimental conditions.
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Is it possible to investigate whether ADCC effectively eliminates cancer cells using linduzumab?
A: Yes, Lintuzumab is designed to enhance ADCC. You can use it in assays that measure natural killer cell activity to assess how effectively it promotes the destruction of CD33-positive cancer cells.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-321-sdAb | Recombinant Anti-human CD33 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-013 | Humanized Anti-CD33 Recombinant Antibody (TAB-013) | IF, IP, Neut, FuncS, ELISA, FC, WB | Humanized (from mouse) IgG4, κ |
| TAB-H37 | Anti-Human CD33 Recombinant Antibody (Gemtuzumab Ozogamicin) | ELISA, FC, IP, FuncS, IF, Neut, IHC | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G008E | Anti-CD33 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G008G | Anti-CD33 immunotoxin (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-L050E | anti-CD33 immunotoxin M195 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L050G | anti-CD33 immunotoxin M195 (IgG)-Gel | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-119LC | Mouse Anti-CD33 Recombinant Antibody (TAB-119LC) | ELISA, IF, RIA | Mouse IgG |
| TAB-120LC | Mouse Anti-CD33 Recombinant Antibody (TAB-120LC) | ELISA, Cyt | Mouse IgG |
| TAB-121LC | Mouse Anti-CD33 Recombinant Antibody (TAB-121LC) | ELISA | Mouse IgG |
| TAB-122LC | Mouse Anti-CD33 Recombinant Antibody (TAB-122LC) | ELISA, Cyt | Mouse IgG |
| TAB-124LC | Mouse Anti-CD33 Recombinant Antibody (TAB-124LC) | ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-123LC | Anti-Human CD33 Recombinant Antibody | FC | Human antibody |
| TAB-146LC | Human Anti-CD33 Recombinant Antibody (TAB-146LC) | ELISA | Human IgG |
| TAB-123LC-S(P) | Anti-Human CD33 Recombinant Antibody scFv Fragment | FC | Human antibody |
| TAB-146LC-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) | ELISA | Human scFv |
| TAB-123LC-F(E) | Anti-Human CD33 Recombinant Antibody Fab Fragment | FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-013LC | Recombinant Anti-Human CD33 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-341CQ | Mouse Anti-CD33 Recombinant Antibody (clone WM53) | FC, IHC-Fr, ICC, IP, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0553 | Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-471CQ | Anti-Human CD33 Recombinant Antibody (TAB-471CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0087-FY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-0087-FY-F(E)) | ELISA | Human Fab |
| HPAB-1981LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1981LY-F(E)) | FC | Humanized Fab |
| HPAB-1982LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1982LY-F(E)) | FC | Humanized Fab |
| HPAB-1983LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1983LY-F(E)) | FC | Humanized Fab |
| HPAB-1984LY-F(E) | Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1984LY-F(E)) | FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-471CQ | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-471CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-013 | Afuco™ Anti-CD33 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-013) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1910LY | Human Anti-CD33 Recombinant Antibody (HPAB-1910LY) | FC, Inhib, FuncS | Humanized IgG |
| HPAB-1911LY | Human Anti-CD33 Recombinant Antibody (HPAB-1911LY) | FC, SPR, FuncS | Humanized IgG |
| HPAB-1912LY | Human Anti-CD33 Recombinant Antibody (HPAB-1912LY) | FC | Humanized IgG |
| HPAB-1913LY | Human Anti-CD33 Recombinant Antibody (HPAB-1913LY) | FC, SPR | Humanized IgG |
| HPAB-1914LY | Human Anti-CD33 Recombinant Antibody (HPAB-1914LY) | FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1910LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1910LY-S(P)) | FC | Humanized scfv |
| HPAB-1911LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1911LY-S(P)) | FC | Humanized scfv |
| HPAB-1912LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1912LY-S(P)) | FC | Humanized scfv |
| HPAB-1913LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1913LY-S(P)) | FC | Humanized scfv |
| HPAB-1914LY-S(P) | Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-1914LY-S(P)) | FC | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC24 | Rabbit Anti-CD33 Antibody (VS-0924-YC24) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY35 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone HIM3-4) | FC | Mouse IgG1, kappa |
| VS-0225-XY36 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone P67.6) | FC | Mouse IgG1, kappa |
| VS-0225-XY37 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (clone WM53) | FC | Mouse IgG1, kappa |
| VS13-YC160 | CytoStream™ Mouse Anti-CD33 Recombinant Antibody (VS13-YC160) | IHC-P, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY84 | Mouse Anti-CD33 scFv-Fc Chimera (VS-0425-FY84) | FC, IF | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC365 | Recombinant Anti-CD33 Vesicular Antibody, EV Displayed (VS-0425-YC365) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1159 | Anti-CD33 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1160 | Anti-Human CD33 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC28 | Recombinant Anti-CD33 (IgV domain x IgC domain) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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