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    Rat Anti-CD44 Recombinant Antibody (clone 9A4); Fab Fragment (CAT#: HPAB-N0328-YC-F(E))

    The antibody (9A4) is a rat monoclonal antibody to CD44, variant 6. This antibody reacts with mouse. The CD44, variant 6 Antibody (9A4) has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
    Figure 3 IHC staining of human vagina
    Figure 3 IHC staining of human vagina
    (Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
    Normal Tissue
    Figure 4 Colon
    Figure 4 Colon
    (Endocrine cells
    Staining: High
    Intensity: Strong
    Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Enterocytes
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Goblet cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Mucosal lymphoid cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Peripheral nerve/ganglion
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Distal tubules
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Proximal tubules (cell body)
    Staining: Medium
    Intensity: Strong
    Quantity: <25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
    Figure 6 Skin
    Figure 6 Skin
    (Cells in basal layer
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Cells in spinous layer
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Fibrohistiocytic cells
    Staining: High
    Intensity: Strong
    Langerhans cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Lymphocytes
    Staining: High
    Intensity: Strong
    Melanocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
    Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
    Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
    Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44

    Specifications

    • Host Species
    • Rat
    • Type
    • Rat Fab
    • Specificity
    • Human CD44
    • Species Reactivity
    • Human, Mouse
    • Clone
    • 9A4
    • Applications
    • WB, ELISA, IHC-P, IHC-Fr

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE and HPLC analysis
    • Buffer
    • PBS
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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