Mouse Anti-MUC1 Recombinant Antibody (TAB-418MZ) (CAT#: TAB-418MZ)
This product is a recombinant mouse antibody that recognizes MUC1 (Mucin1). The original antibody was secreted by the hybridoma and the recombinant antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. This antibody is against the immunogenic glycopeptide of MUC1.
Specific Inquiry
DB
Figure 1 Anti-Human MUC1 Therapeutic Antibody (TAB-418MZ) in DB
Dot Blot analysis of TAB-418MZ was performed by coating with human MUC1 protein (His tag).
TAB-418MZ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG was used as a secondary antibody (1: 2000)
WB
Figure 2 Anti-Human MUC1 Therapeutic Antibody (TAB-418MZ) in WB
Western blot analysis of TAB-418MZ was performed with human MUC1 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-418MZ and HRP goat anti-mouse IgG was used as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
SDS-PAGE
Figure 3 Mouse Anti-MUC1 Recombinant Antibody (5E5) (TAB-418MZ) in SDS-PAGE
SDS-PAGE analysis of TAB-418MZ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-418MZ was greater than 95% as determined by SDS-PAGE
SEC-HPLC
Figure 4 Mouse Anti-MUC1 Recombinant Antibody (5E5) (TAB-418MZ) in HPLC
The purity of TAB-388CT was greater than 93% as determined by SEC-HPLC.
Specifications
- Immunogen
- The fully GalNAc-glycosylated 60-mer MUC1 glycopeptide coupled to KLH
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MUC1 (Mucin1)
- Species Reactivity
- Human
- Applications
- ELISA, FuncS, IHC, IF, FC, ADCC
- Related Disease
- Breast carcinoma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MUC1; mucin 1, cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant;
- Gene ID
- 4582
- UniProt ID
- P15941
Related Resources
Kvorjak, Michael, et al. "Cross-talk between colon cells and macrophages increases ST6GALNAC1 and MUC1-sTn expression in ulcerative colitis and colitis-associated colon cancer." Cancer immunology research 8.2 (2020): 167-178.
The study investigated the interaction between colon cells and macrophages in the context of ulcerative colitis (UC) and colitis-associated colon cancer (CACC). Researchers found that macrophages, particularly the M2-like phenotype, play a significant role in promoting the expression of the glycosyltransferase ST6GALNAC1 in colon cancer cells. This enzyme catalyzes the addition of sialic acid to O-linked GalNAc residues, leading to the formation of tumor-associated sialyl-Tn (sTn) antigens on the mucin MUC1. The study demonstrated that the presence of M2 macrophages, through cytokines such as IL13 and CCL17, increased the expression of ST6GALNAC1 and the corresponding MUC1-sTn glycoform, contributing to the pathogenesis and progression of UC and CACC.
Creative Biolabs provided the anti-MUC1 antibody 5E5 (Cat#: TAB-418MZ) used in the study. This antibody was crucial for identifying and quantifying the MUC1-sTn glycoform in tissue samples and cell cultures. By enabling the detection of specific glycoforms of MUC1, the antibody facilitated the evaluation of macrophage-induced changes in glycosylation patterns, thus playing a vital role in elucidating the mechanisms through which macrophages influence colon cancer progression.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
Mouse Antibody
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Fc Glycosylation
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ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP504-YC) | FC | Camel VHH |
| HPAB-AP505-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP505-YC) | ELISA | Camel VHH |
| HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0736-YJ-VHH) | ELISA | Camelid VHH |
| HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0737-YJ-VHH) | ELISA | Camelid VHH |
Customer Reviews and Q&As
Customer Review
Q&As
-
Great for MUC1 Detection
The Anti-MUC1 Recombinant Antibody has been excellent for our MUC1 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time. -
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for MUC1 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab. -
Consistent and High-Quality
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for MUC1 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-418MZ, RRID: AB_2941837)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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