Z-domain of protein A scaffold protein anti-Human CD25 (CAT#: SZA-L215)

This CD25 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=10 pM) for CD25 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.


Specific Inquiry
  • Size:
  • Conjugation:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Normal Tissue
RNA Expression

Specifications

  • Scaffold Name
  • Z-domain of protein A
  • Origin
  • Staphylococcal protein A
  • Core Structure
  • 3-α helixes
  • Variable Regions
  • 13 Residues on first and second helix surface
  • Target
  • CD25
  • Species Reactivity
  • Human
  • Expression Host
  • E. coli
  • Affinity Constant
  • 10 pM
  • Applications
  • ELISA; IHC; Microscopy; FC; WB; FuncS

Target

  • Alternative Names
  • Staphylococcal protein A; Z-domain of protein A; IL2RA; interleukin 2 receptor, alpha; p55; CD25; IL2R; TCGFR; IDDM10; interleukin-2 receptor subunit alpha; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; scFv; Hodgkin's lymphoma; lymphoma; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "IL2RA"

Intrabody

CAT Product Name Application Type
IAB-B073(A) Recombinant Anti-human IL2RA Intrabody [(D-Arg)9] IF, ELISA, FuncS scFv-(D-Arg)9
IAB-B073(G) Recombinant Anti-human IL2RA Intrabody [+36 GFP] IF, FC, WB, FuncS scFv-(+36GFP)
IAB-B073(T) Recombinant Anti-human IL2RA Intrabody [Tat] WB, CO-IP, FuncS scFv-Tat

Chimeric Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-L027D anti-IL2RA immunotoxin RFT5 (scFv)-DT Cytotoxicity assay, Functional assay
AGTO-L027R anti-IL2RA immunotoxin RFT5 (scFv)-RTA Cytotoxicity assay, Functional assay
AGTO-L028E anti-IL2RA immunotoxin RFT5 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L028D anti-IL2RA immunotoxin RFT5 (IgG)-DT Cytotoxicity assay, Functional assay
AGTO-L028R anti-IL2RA immunotoxin RFT5 (IgG)-RTA Cytotoxicity assay, Functional assay

Neutralizing Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-1350CQ Mouse Anti-IL2RA Recombinant Antibody (clone 2R12) Block, FC, ICC, IF, IHC, IP, WB Mouse IgG1
NEUT-1356CQ Rat Anti-Il2ra Recombinant Antibody (VMC199) FC, IP, IHC, Block, Depletion Rat IgG1, λ
NEUT-1357CQ Rat Anti-Il2ra Recombinant Antibody (clone 3C7) FC, IP, Inhib Rat IgG2b, κ
NEUT-1358CQ Rat Anti-Il2ra Recombinant Antibody (clone mAb0817) Block, Depletion Rat IgG1
NEUT-1359CQ Rat Anti-Il2ra Recombinant Antibody (clone mAb0845) Block, FC, IHC-Fr, IP Rat IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1808 Rabbit Anti-IL2RA Recombinant Antibody (clone DS1808AB) IHC, ICC, IP Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
FAMAB-0197CQ Mouse Anti-IL2RA Recombinant Antibody (clone 2C8) ELISA, WB, FC Mouse IgG1, κ
FAMAB-0198CQ Human Anti-IL2RA Recombinant Antibody (clone 4C1) ELISA, WB, FC Chimeric (mouse/human) IgG1, κ
FAMAB-0199CQ Mouse Anti-IL2RA Recombinant Antibody (clone 4F8) ELISA, WB, FC Mouse IgG2a, κ
FAMAB-0200CQ Human Anti-IL2RA Recombinant Antibody (clone huD1) ELISA, FC, Block Humanized IgG1, κ
FAMAB-0201CQ Mouse Anti-IL2RA Recombinant Antibody (clone D1) ELISA, FC, Block Mouse IgG2a, κ

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-004 Afuco™ Anti-IL2RA ADCC Recombinant Antibody (Basiliximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-023 Afuco™ Anti-IL2RA ADCC Recombinant Antibody (Daclizumab), ADCC Enhanced ELISA, Neut, IF, IP, FC, FuncS ADCC enhanced antibody
AFC-TAB-155 Afuco™ Anti-IL2RA ADCC Recombinant Antibody (Inolimomab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for SZA-L215. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare