Z-domain of protein A scaffold protein anti-Human CD25

Name: Z-domain of protein A scaffold protein anti-Human CD25
SKU: SZA-L215
Rating: 5 (1 reviews)

CAT#: SZA-L215

This CD25 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=10 pM) for CD25 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54622/137874_A_8_8.jpg

Normal Tissue Figure 2 Tonsil

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54622/137874_A_4_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134460-IL2RA

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Specifications

Scaffold Name
Z-domain of protein A

Origin
Staphylococcal protein A

Core Structure
3-α helixes

Variable Regions
13 Residues on first and second helix surface

Target
CD25

Species Reactivity
Human

Expression Host
E. coli

Affinity Constant
10 pM

Applications
ELISA; IHC; Microscopy; FC; WB; FuncS

Target

Alternative Names
Staphylococcal protein A; Z-domain of protein A; IL2RA; interleukin 2 receptor, alpha; p55; CD25; IL2R; TCGFR; IDDM10; interleukin-2 receptor subunit alpha; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; scFv; Hodgkin's lymphoma; lymphoma; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

Gene ID
3559

UniProt ID
P01589

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Datasheet

Datasheet - SZA-L215.pdf

MSDS

MSDS - SZA-L215.pdf

COA

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