Recombinant Anti-human IL2RA Intrabody [(D-Arg)₉]

Name: Recombinant Anti-human IL2RA Intrabody [(D-Arg)9]
SKU: IAB-B073(A)
Rating: 5 (1 reviews)

CAT#: IAB-B073(A)

Recombinant anti-IL2RA intrabody linked to (D-Arg)₉ works within cells to bind human IL2RA, expressed in E. coli cells.

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54622/137874_A_8_8.jpg

Normal Tissue Figure 2 Tonsil

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54622/137874_A_4_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134460-IL2RA

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Specifications

Immunogen
human interleukin 2 receptor, alpha

Host Species
mouse

Type
scFv-(D-Arg)₉

Cell Penetrating Peptide
(D-Arg)₉
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.

Species Reactivity
human

Applications
Can be useful in applications such as: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies

Product Property

Purity
90–95% (SDS-PAGE)

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

Alternative Names
IL2RA; interleukin 2 receptor, alpha; p55; CD25; IL2R; TCGFR; IDDM10; interleukin-2 receptor subunit alpha; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha

Gene ID
3559

UniProt ID
P01589

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - IAB-B073(A).pdf

MSDS

MSDS - IAB-B073(A).pdf

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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