anti-IL2RA immunotoxin RFT5 (scFv)-RTA

Name: anti-IL2RA immunotoxin RFT5 (scFv)-RTA
SKU: AGTO-L027R
Rating: 5 (1 reviews)

CAT#: AGTO-L027R

This immunotoxin was achieved by conjugating/fusing the Anti-IL2RA scFv to ricin A chain. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to Hodgkin's lymphoma cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of Hodgkin's lymphoma.

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54622/137874_A_8_8.jpg

Normal Tissue Figure 2 Tonsil

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54622/137874_A_4_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134460-IL2RA

❮

❯

Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
RFT5

Molecule Class
scFv-RTA

Toxin Description
Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
Hodgkin's lymphoma

Target

Alternative Names
IL2RA; interleukin 2 receptor, alpha; p55; CD25; IL2R; TCGFR; IDDM10; interleukin-2 receptor subunit alpha; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; scFv; Hodgkin's lymphoma; lymphoma; Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA

Gene ID
3559

UniProt ID
P01589

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for AGTO-L027R. Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-L027R.pdf

MSDS

MSDS - AGTO-L027R.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone RFT5"

CAT
Product Name

See other products for "IL2RA"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1094z	Mouse Anti-IL2RA Recombinant Antibody (clone 18C9)	WB, ELISA, IF, IHC, FuncS	Mouse IgG1, κ
VS3-CJ266	Mouse Anti-IL2RA Recombinant Antibody (VS3-CJ266)	WB, IHC, ELISA
VS3-CJ267	Mouse Anti-IL2RA Recombinant Antibody (clone 7G7B6)	IF, FC	Mouse IgG2a
VS3-WK1278	Rat Anti-IL2RA Recombinant Antibody (clone PC-61.5.3)	IF, FC	Rat IgG1, κ
VS3-WK1456	Mouse Anti-IL2RA Recombinant Antibody (clone 776)	FC, IF	Mouse IgG2a

CAT	Product Name	Application	Type
IAB-B073(A)	Recombinant Anti-human IL2RA Intrabody [(D-Arg)₉]	IF, ELISA, FuncS	scFv-(D-Arg)₉
IAB-B073(G)	Recombinant Anti-human IL2RA Intrabody [+36 GFP]	IF, FC, WB, FuncS	scFv-(+36GFP)
IAB-B073(T)	Recombinant Anti-human IL2RA Intrabody [Tat]	WB, CO-IP, FuncS	scFv-Tat

CAT	Product Name	Application	Type
TAB-004	Anti-Human CD25 Recombinant Antibody (Basiliximab)	IF, IP, Neut, FuncS, ELISA, FC, ICC	IgG1 - kappa
PABX-030	Recombinant Human Anti-CD25 Antibody (SDZCHII621)	Neut, FuncS	IgG
PABX-030-F (E)	Recombinant Mouse Anti-CD25 Antibody Fab Fragment (SDZCHII621)	Neut, FuncS	Fab

CAT	Product Name	Application	Type
TAB-155	Anti-Human CD25 Recombinant Antibody (TAB-155)	IP, IF, FuncS, FC, Neut, ELISA, ICC	IgG1 - kappa
TAB-641LC	Anti-human IL2RA Recombinant Antibody (TAB-641LC)	ELISA
TAB-641LC-S(P)	Anti-human IL2RA scFv Fragment (TAB-641LC-S(P))	ELISA
TAB-641LC-F(E)	Anti-human IL2RA Fab Fragment (TAB-641LC-F(E))	ELISA
PABX-029-S (P)	Recombinant Human Anti-CD25 Antibody scFv Fragment (Daclizumab)	WB, ELISA, FuncS	scFv

CAT	Product Name	Application
AGTO-L027D	anti-IL2RA immunotoxin RFT5 (scFv)-DT	Cytotoxicity assay, Functional assay
AGTO-L028E	anti-IL2RA immunotoxin RFT5 (IgG)-PE	Cytotoxicity assay, Functional assay
AGTO-L028D	anti-IL2RA immunotoxin RFT5 (IgG)-DT	Cytotoxicity assay, Functional assay
AGTO-L028R	anti-IL2RA immunotoxin RFT5 (IgG)-RTA	Cytotoxicity assay, Functional assay
AGTO-L078E	IL2-PE immunotoxin	Cytotoxicity assay, Functional assay

CAT	Product Name	Application	Type
PSBL-242	Human Anti-IL2RA Recombinant Antibody (clone 1H4); scFv Fragment	WB, IHC, FuncS	Human scFv
PSBZ-020	Mouse Anti-IL2RA Recombinant Antibody (clone CHI621); scFv Fragment	Block	Mouse scFv
PSBZ-082	Mouse Anti-IL2RA Recombinant Antibody (clone SDZ CHI621); scFv Fragment	Block	Mouse scFv
HPAB-0060-FY-S(P)	Human Anti-IL2RA Recombinant Antibody; scFv Fragment (HPAB-0060-FY-S(P))	ELISA	Humanized scFv
HPAB-0197-FY-S(P)	Mouse Anti-IL2RA Recombinant Antibody (clone WuTac); scFv Fragment	FC, DB, WB	Mouse scFv

CAT	Product Name	Application	Type
PFBL-608	Human Anti-IL2RA Recombinant Antibody (clone RTF5); Fab Fragment	WB, FuncS	Chimeric (mouse/human) Fab
HPAB-0559-WJ-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (HPAB-0559-WJ-F(E))	ELISA, FC	Human Fab
HPAB-0560-WJ-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (HPAB-0560-WJ-F(E))	ELISA, FC	Human Fab
HPAB-0561-WJ-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (HPAB-0561-WJ-F(E))	ELISA, FC	Human Fab
HPAB-1536WJ-F(E)	Rat Anti-IL2RA Recombinant Antibody; Fab Fragment (clone YTH 906.9.21)	ELISA, WB	Rat Fab

CAT	Product Name	Application	Type
TAB-642LC-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (TAB-642LC-F(E))	ELISA, FC	Human Fab
TAB-643LC-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (TAB-643LC-F(E))	ELISA, FC	Human Fab
TAB-644LC-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (TAB-644LC-F(E))	ELISA, FC	Human Fab
TAB-645LC-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (TAB-645LC-F(E))	ELISA, FC	Human Fab
PABX-029	Recombinant Human Anti-CD25 Antibody (Daclizumab)	WB, ELISA, FuncS, FC, Cyt	IgG

CAT	Product Name	Application	Type
TAB-646LC-F(E)	Anti-human IL2RA Fab Fragment (48M54S)	ELISA, FC	Humanized antibody
TAB-647LC-F(E)	Anti-human IL2RA Fab Fragment (48M51L)	ELISA, FC	Humanized antibody
TAB-648LC-F(E)	Anti-human IL2RA Fab Fragment (48V54S)	ELISA, FC	Humanized antibody
TAB-649LC-F(E)	Anti-human IL2RA Fab Fragment (Dmab)	FC	Humanized antibody
TAB-650LC-F(E)	Human Anti-IL2RA Recombinant Antibody; Fab Fragment (TAB-650LC-F(E))	ELISA	Humanized Fab

CAT	Product Name	Application	Type
NEUT-1344CQ	Mouse Anti-IL2RA Recombinant Antibody (clone B-B10)	FC, Neut, WB, IF	Mouse IgG1, κ
NEUT-1345CQ	Mouse Anti-IL2RA Recombinant Antibody (clone CBL017)	Neut, WB, IHC	Mouse IgG1
NEUT-1346CQ	Mouse Anti-IL2RA Recombinant Antibody (clone CBL242)	ELISA, FC, Neut	Mouse IgG1, κ
NEUT-1348CQ	Mouse Anti-IL2RA Recombinant Antibody (clone CBL726)	WB, IHC, ICC, Neut	Mouse IgG1
NEUT-1349CQ	Mouse Anti-IL2RA Recombinant Antibody (NEUT-1349CQ)	IHC, Neut, WB	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-1350CQ	Mouse Anti-IL2RA Recombinant Antibody (NEUT-1350CQ)	Block, FC, ICC, IF, IHC, IP, WB	Mouse IgG1
NEUT-1356CQ	Rat Anti-Il2ra Recombinant Antibody (VMC199)	FC, IP, IHC, Block, Depletion	Rat IgG1, λ
NEUT-1357CQ	Rat Anti-Il2ra Recombinant Antibody (clone 3C7)	FC, IP, Inhib	Rat IgG2b, κ
NEUT-1358CQ	Rat Anti-Il2ra Recombinant Antibody (NEUT-1358CQ)	Block, Depletion	Rat IgG1
NEUT-1359CQ	Rat Anti-Il2ra Recombinant Antibody (NEUT-1359CQ)	Block, FC, IHC-Fr, IP	Rat IgG1

CAT	Product Name	Application	Type
MOR-1808	Rabbit Anti-IL2RA Recombinant Antibody (clone DS1808AB)	IHC, ICC, IP	Rabbit IgG

CAT	Product Name	Application	Type
AFC-TAB-004	Afuco™ Anti-IL2RA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-004)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-023	Afuco™ Anti-IL2RA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-023)	ELISA, Neut, IF, IP, FC, FuncS	ADCC enhanced antibody
AFC-TAB-155	Afuco™ Anti-IL2RA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-155)	IP, IF, FuncS, FC, Neut, ELISA	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0424-XY150	AbPlus™ Anti-IL2RA Magnetic Beads (2R12)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC133	Human Anti-IL2RA Recombinant Antibody Hexamer (VS-0924-YC133), CDC Enhanced	CDC	Antibody hexamer

CAT	Product Name	Application	Type
VS-0924-YC47	Rabbit Anti-IL2RA Antibody (VS-0924-YC47) - Cancer Stem Cell Marker	IHC	Rabbit IgG

CAT	Product Name	Application	Type
VS-0125-FY44	Human Anti-IL2RA (clone MAK179) scFv-Fc Chimera	Inhib	Human IgG1, scFv-Fc
VS-0425-FY42	Mouse Anti-IL2RA (clone mAb1) scFv-Fc Chimera	ELISA	Mouse IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY135	CytoStream™ Mouse Anti-IL2RA Recombinant Antibody (clone BC96)	FC	Mouse IgG1, kappa

CAT	Product Name	Application	Type
VS-0425-YC386	Recombinant Anti-IL2RA Vesicular Antibody, EV Displayed (VS-0425-YC386)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application
VS-0525-XY3520	Anti-IL2RA Immunohistochemistry Kit	IHC
VS-0525-XY3521	Anti-Mouse IL2RA Immunohistochemistry Kit	IHC
VS-0525-XY3522	Anti-Rat IL2RA Immunohistochemistry Kit	IHC

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Mouse Anti-CD3E Recombinant Antibody (clone UCHT1); Fab Fragment (DrMAB-989) in HPLC

Mouse Anti-CD3E Recombinant Antibody (clone UCHT1); Fab Fragment (CAT# DrMAB-989)
Application: FC, Cyt, Stim, PP, Agonist

Figure 2 Human IL1 Beta Therapeutic Antibody (TAB-021) in WB

Anti-Human IL1Beta Recombinant Antibody (Canakinumab) (CAT# TAB-021)
Application: WB, ELISA, IP, FC, FuncS, Neut, IF

Figure 2 Anti-Human DR5 Recombinant Antibody (TAB-203) in WB

Human Anti-TNFRSF10B Recombinant Antibody (TAB-203) (CAT# TAB-203)
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC

Figure 2 Human Anti-ERBB3 Recombinant Antibody (TAB-189) in ELISA

Human Anti-ERBB3 Recombinant Antibody (TAB-189) (CAT# TAB-189)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Figure 3 Anti-GPC3 Antibody (TAB-H14) in ELISA

Anti-Human GPC3 Recombinant Antibody (Codrituzumab) (CAT# TAB-H14)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC

Figure 4 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in ELISA

Anti-Human IL1 beta Recombinant Antibody (TAB-161) (CAT# TAB-161)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 1 Mouse Anti-GD2 Recombinant Antibody (PABL-101) in SDS-PAGE

Mouse Anti-GD2 Recombinant Antibody (clone 3F8) (CAT# PABL-101)
Application: FC, ELISA, FuncS

Figure 11 Anti-IAV HA Recombinant Antibody (PABL-214) in DB

Recombinant Human Anti-IAV HA Antibody (FI6V3) (CAT# PABL-214)
Application: WB, IHC, FuncS

Figure 2 Human Anti-VEGF Recombinant Antibody (PABC-131) in ELISA

Human Anti-VEGF Recombinant Antibody (clone B20-4) (CAT# PABC-131)
Application: ELISA, Neut, Block

Figure 2 Anti-Human CD24 Recombinant Antibody (TAB-006LC) in WB

Mouse Anti-CD24 Recombinant Antibody (TAB-006LC) (CAT# TAB-006LC)
Application: FC, FuncS

Figure 6 Anti-Human CD5 Recombinant Antibody (TAB-308LC) in FACS

Anti-Human CD5 Recombinant Antibody (5D7) (CAT# TAB-308LC)
Application: Cyt, FC, FuncS

Figure 4 Human Anti-TSHR Recombinant Antibody (TAB-191CQ) in ELISA

Human Anti-TSHR Recombinant Antibody (TAB-191CQ) (CAT# TAB-191CQ)
Application: ELISA, WB, Inhib

Figure 2 Anti-SARS-CoV S Recombinant Antibody (MRO-654LC) in HPLC

Mouse Anti-SARS-CoV S Recombinant Antibody (MRO-654LC) (CAT# MRO-654LC)
Application: ELISA

Figure 4 Anti-Human C5 Recombinant Antibody (TAB-453CQ) in WB

Anti-Human C5 Recombinant Antibody (TAB-453CQ) (CAT# TAB-453CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 4 Anti-Human CD40 Recombinant Antibody (Selicrelumab) (TAB-470CQ) in ELISA

Anti-Human CD40 Recombinant Antibody (Selicrelumab) (CAT# TAB-470CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 2 Human Anti-CD70 Recombinant Antibody (HPAB-0102-LSX) in HPLC

Human Anti-CD70 Recombinant Antibody (HPAB-0102-LSX) (CAT# HPAB-0102-LSX)
Application: ELISA, FuncS

Figure 3 Human Anti-HIV-1 gp160 Recombinant Antibody (MRO-3786CQ) in ELISA

Human Anti-HIV-1 gp160 Recombinant Antibody (MRO-3786CQ) (CAT# MRO-3786CQ)
Application: FuncS

Figure 2 Recombinant Mouse Anti-CD22 Antibody (HPAB-N0324-YC) in HPLC

Mouse Anti-CD22 Recombinant Antibody (HPAB-N0324-YC) (CAT# HPAB-N0324-YC)
Application: FuncS

Figure 2 Mouse Anti-FSTL3 Recombinant Antibody (HPAB-N0094-YC) in HPLC

Mouse Anti-FSTL3 Recombinant Antibody (HPAB-N0094-YC) (CAT# HPAB-N0094-YC)
Application: Neut, WB

Figure 2 Recombinant Mouse Anti-Psa Fab Fragment (HPAB-3383LY-F(E)) in HPLC

Mouse Anti-Psa Recombinant Antibody; Fab Fragment (HPAB-3383LY-F(E)) (CAT# HPAB-3383LY-F(E))
Application: ELISA

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.