Human Anti-CD40 Recombinant Antibody (clone 21.4.1)
CAT#: HPAB-1834-FY
This product is a recombinant antibody that recognizes CD40. The monoclonal antibody 21.4.1 reacts with the epitope of CD40. 21.4.1 has direct anti-tumor activity against CD40(+) tumors. This anti-tumor activity for antibody 21.4.1 was enhanced when immune cells were present.
Published Data
Gene Expression
Activ
Figure 1 CH1-hinge basis of agonistically inactive IgG3 and superior IgG2.
Representative FACS profile showing the percentage of OT-I cells (CD45.1+TCRVα2+) among CD8+ T cells in human CD40/FcγR-transgenic mice treated and analyzed together with control or anti-human CD40 antibodies of indicated clones (Clones 21.4.1 and 3.1.1 have been described in Patent No.:US 7,338,660; Clone 21.4.1 in the IgG2 form is also known as CP-870,893) and constant domains (30 μg per mouse).
Liu, X., Zhao, Y., Shi, H., Zhang, Y., Yin, X., Liu, M., ... & Li, F. (2019). Human immunoglobulin G hinge regulates agonistic anti-CD40 immunostimulatory and antitumour activities through biophysical flexibility. Nature communications, 10(1), 1-15.
Activ
Figure 2 CH1-hinge basis of agonistically inactive IgG3 and superior IgG2.
Representative quantification showing the percentage of OT-I cells (CD45.1+TCRVα2+) among CD8+ T cells in human CD40/FcγR-transgenic mice treated and analyzed together with control or anti-human CD40 antibodies of indicated clones (Clones 21.4.1, Clone 21.4.1 in the IgG2 form is also known as CP-870,893) and constant domains (30 μg per mouse).
Liu, X., Zhao, Y., Shi, H., Zhang, Y., Yin, X., Liu, M., ... & Li, F. (2019). Human immunoglobulin G hinge regulates agonistic anti-CD40 immunostimulatory and antitumour activities through biophysical flexibility. Nature communications, 10(1), 1-15.
Agonist
Figure 3 Shows a dose-response curve that illustrates the ability of an anti-CD40 antibody of the invention (21.4.1) to enhance IL-12p40 production by human dendritic cells.
Agonist
Figure 4 Shows a dose-response curve that illustrates the ability of an anti-CD40 antibody of the invention (21.4.1) to enhance IL-12p70 production by human dendritic cells.
Inhib
Figure 5 Shows a tumor growth inhibition curve that illustrates the reduced growth of CD40 positive Daudi tumors in SCID-beige mice treated with an anti-CD40 antibody of the invention (21.4.1).
WB
Figure 6 Shows a Western blot analysis of anti-CD40 agonist antibodies to reduced (R) and non-reduced (NR) human CD40.
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Normal Tissue
Figure 1 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72868/156401_A_8_8.jpg
Normal Tissue
Figure 2 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31567/156626_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, EFO-21)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101017-CD40
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human CD40
- Species Reactivity
- Human, Cynomolgus monkey
- Clone
- 21.4.1
- Applications
- Activ, Agonist, Inhib, WB
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CD40 antibody has been reported in applications of Activation, Agonist, Inhibition, Western Blot.
Target
REVIEWS AND Q&AS
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Cost-effective
This antibody product offers great quality at a cost-effective price point. We have been able to stay within budget without compromising on the integrity of our research.
Reliable service
The customer service is very reliable. We received prompt responses to our inquiries and quick replacements when there were issues with our shipments.
High specificity
The specificity of the antibodies in this product is impressive. It has allowed us to target particular antigens with high precision in our assays.
Q&As
-
How do I know if an antibody is suitable for my species of interest?
A: Check the product information for species reactivity. If unsure, contact the manufacturer or perform a pilot experiment.
-
What is the shelf life of an antibody after it's been reconstituted?
A: Typically, reconstituted antibodies are stable for several weeks at 4°C, but it's best to follow the manufacturer's guidelines.
-
Can I freeze an aliquoted antibody that was previously stored at 4°C?
A: It depends on the antibody. Some can be frozen without issue, while others may lose activity.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-1834-FY, RRID: AB_3111422)
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Related Diseases
Asthma
Systemic Lupus Erythematosus
Primary Immunodeficiency
Allograft Rejection
Autoimmune Thyroid Disease
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| NEUT-365CQ | Hamster Anti-CD40 Recombinant Antibody (clone HM40-3) | FC, Block, Costim, IHC | Hamster IgM |
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| HPAB-0165-YC-S(P) | Human Anti-CD40 Recombinant Antibody; scFv Fragment (HPAB-0165-YC-S(P)) | FC | Human scFv |
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