Mouse Anti-CD44 Recombinant Antibody (ZG-0115J)
CAT#: ZG-0115J
This product is a mouse antibody that recognizes CD44.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-CD44 Recombinant Antibody (ZG-0115J) in IHC
Immunohistochemical analysis of paraffin-embedded Transitional cell carcinoma. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-CD44 Recombinant Antibody (ZG-0115J) in IHC
Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid , pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
WB
Figure 3 Mouse Anti-CD44 Recombinant Antibody (ZG-0115J) in WB
Western blot analysis of CD44 Antibody at 1:1000 dilution.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/203_H10_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/112/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human vagina
Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/316/5_B_2_2_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_8_5.jpg
Normal Tissue
Figure 6 Skin
Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity:>75%
Lymphocytes
Staining: High
Intensity: Strong
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_B_7_1.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5785/16384_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026508-CD44
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Specifications
- Immunogen
- Synthesized peptide derived from human CD44
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD44
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
- Alternative Names
- IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; CD44 antigen; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Indian blood group antigen; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; epican; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1; soluble CD44
- Gene ID
- 960
- UniProt ID
- P16070
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Customer Review
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Highly recommended
I highly recommend this antibody product for any lab looking to enhance their research. The quality and support provided are exceptional.
Outstanding performance
The performance of this antibody product has been outstanding, significantly contributing to the success of our recent projects.
Clear instructions
The product comes with clear and concise instructions, making it easy for even less experienced lab members to use effectively.
Q&As
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What should I do when antibodies don't function as they should?
A: Verify that everything is kept properly, examine your routines, and think about contacting the manufacturer for assistance.
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How can I tell whether an antibody is suitable for the species I want to study?
A: See the product specifications for additional data regarding species reactivity. Have the manufacturer do a test if you are unsure.
-
What is the half-life of antibodies upon reconstitution?
A: Reconstituted antibodies can often be kept stable for several weeks at 4 °C, although it is important to follow the manufacturer's recommendations.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| TAB-231LC-S(P) | Mouse Anti-CD44 Recombinant Antibody; scFv Fragment (TAB-231LC-S(P)) | ELISA | Mouse scFv |
| TAB-232LC-S(P) | Mouse Anti-CD44 Recombinant Antibody; scFv Fragment (TAB-232LC-S(P)) | ELISA | Mouse scFv |
| TAB-231LC-F(E) | Human Anti-CD44 Recombinant Antibody; Fab Fragment (TAB-231LC-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| TAB-232LC-F(E) | Human Anti-CD44 Recombinant Antibody; Fab Fragment (TAB-232LC-F(E)) | ELISA | Chimeric (mouse/human) Fab |
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|---|---|---|---|
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| ZG-0590J | Rabbit Anti-CD44 Recombinant Antibody (clone 4B7) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX190 | Mouse Anti-CD44 Recombinant Antibody (clone 2H12-E7-G7) | WB, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0101MZ | Chicken Anti-CD44 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-378CQ | Rat Anti-CD44 Recombinant Antibody (clone 1M7.8.1) | FC, IF, Inhib, IP, WB | Rat IgG2b |
| NEUT-381CQ | Human Anti-CD44 Recombinant Antibody (clone Hermes-3) | ELISA, FC, IF, IHC, IP, Neut, WB | Human IgG1, κ |
| NEUT-382CQ | Rat Anti-CD44 Recombinant Antibody (clone IM7) | Block | Rat IgG2b |
| NEUT-383CQ | Rat Anti-CD44 Recombinant Antibody (clone KM201), FITC | Block, FC, IP, WB, FuncS | Rat IgG1, κ |
| NEUT-384CQ | Rat Anti-CD44 Recombinant Antibody (clone KM81) | IF, IHC-P, FC, Block | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-379CQ | Mouse Anti-CD44 Recombinant Antibody (clone F10-44-2) | WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC | Mouse IgG2a |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0019-CN-S(P) | Mouse Anti-CD44 Recombinant Antibody (clone AV6); scFv Fragment | FC | Mouse scFv |
| HPAB-0746-FY-F(E) | Human Anti-CD44 Recombinant Antibody; Fab Fragment (HPAB-0746-FY-F(E)) | ELISA | Human Fab |
| HPAB-0747-FY-F(E) | Human Anti-CD44 Recombinant Antibody; Fab Fragment (HPAB-0747-FY-F(E)) | ELISA | Human Fab |
| HPAB-0563-WJ-S(P) | Human Anti-CD44 Recombinant Antibody (clone 1A9); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0564-WJ-S(P) | Human Anti-CD44 Recombinant Antibody (clone 2D1); scFv Fragment | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0307-CN | Mouse Anti-CD44 Recombinant Antibody (clone A7-1) | WB, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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