Human Anti-CD70 Recombinant Antibody (clone c1F6) (CAT#: HPAB-AP585-YC)

Figure 1 c1F6 mediates ADCC, ADCP, and CDC activity.

Representative flow cytometry analysis and fluorescence microscopy of c1F6-mediated phagocytosis. For flow cytometry, 786-O target cells were labeled with PKH26 lipophilic dye, treated with c1F6 or nonbinding control IgG, then mixed with monocyte-derived macrophages (Mφ). Mφ were stained with AF488-conjugated anti-CD11b. Cells present in the top-right quadrant (PKH26+CD11b+) are Mφ that internalized tumor cells. For microscopy, tumor cells were labeled with PKH67 (green), and the macrophages were detected with Alexa Fluor 568–conjugated antibody specific for CD11b (red). Images were taken with a Leitz Orthoplan Research microscope (100×/1.32 NA objective lens) using a Nikon Coolpix 990 camera (Nikon, Tokyo, Japan), then processed with Adobe Photoshop Elements version 2.0 (Adobe Systems, San Jose, CA).

McEarchern, J. A., Oflazoglu, E., Francisco, L., McDonagh, C. F., Gordon, K. A., Stone, I., ... & Grewal, I. S. (2007). Engineered anti-CD70 antibody with multiple effector functions exhibits in vitro and in vivo antitumor activities. Blood, 109(3), 1185-1192.

Figure 2 c1F6 mediates ADCC, ADCP, and CDC activity.

Representative flow cytometry analysis and fluorescence microscopy of c1F6-mediated phagocytosis. For flow cytometry, 786-O target cells were labeled with PKH26 lipophilic dye, treated with c1F6 or nonbinding control IgG, then mixed with monocyte-derived macrophages (Mφ). Mφ were stained with AF488-conjugated anti-CD11b. Cells present in the top-right quadrant (PKH26+CD11b+) are Mφ that internalized tumor cells. For microscopy, tumor cells were labeled with PKH67 (green), and the macrophages were detected with Alexa Fluor 568–conjugated antibody specific for CD11b (red). Images were taken with a Leitz Orthoplan Research microscope (100×/1.32 NA objective lens) using a Nikon Coolpix 990 camera (Nikon, Tokyo, Japan), then processed with Adobe Photoshop Elements version 2.0 (Adobe Systems, San Jose, CA).

McEarchern, J. A., Oflazoglu, E., Francisco, L., McDonagh, C. F., Gordon, K. A., Stone, I., ... & Grewal, I. S. (2007). Engineered anti-CD70 antibody with multiple effector functions exhibits in vitro and in vivo antitumor activities. Blood, 109(3), 1185-1192.

Figure 3 In vitro and in vivo activity of c1F6 requires functional Fc–Fcγ receptor interaction.

Binding of c1F6 and c1F6v to CD70+ cells was detected with a fluorochrome-conjugated anti–human IgG antibody by flow cytometry. ADCC, CDC, and ADCP activities of c1F6 (solid squares) compared with c1F6v (open diamonds) or nonbinding IgG1 (open circles) were measured.

McEarchern, J. A., Oflazoglu, E., Francisco, L., McDonagh, C. F., Gordon, K. A., Stone, I., ... & Grewal, I. S. (2007). Engineered anti-CD70 antibody with multiple effector functions exhibits in vitro and in vivo antitumor activities. Blood, 109(3), 1185-1192.

Figure 4 Host effector cells are required for in vivo antitumor activity of c1F6.

Raji-bearing mice were depleted of NK cells Mφ, and Neut subsets. After that, mice were either left untreated or treated with 4 mg/kg c1F6 one day following Raji challenge (n = 10 per group). (A) Untreated versus c1F6 (P < .001), untreated versus c1F6 − NK (P = .002), and c1F6 versus c1F6 − NK (P = .021).

McEarchern, J. A., Oflazoglu, E., Francisco, L., McDonagh, C. F., Gordon, K. A., Stone, I., ... & Grewal, I. S. (2007). Engineered anti-CD70 antibody with multiple effector functions exhibits in vitro and in vivo antitumor activities. Blood, 109(3), 1185-1192.