Human Anti-CD80 Recombinant Antibody (clone hu1F1) (CAT#: HPAB-0063-YJ)

This product is a recombinant humanized antibody that recognizes human B7-1. Mab hu1F1 functions in treatments for various autoimmune diseases, transplant rejection, inflammatory disorders, and infectious diseases.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Inhib

Figure 1 Tumor necrosis factor-a stimulated porcine splenocytes activates human T cells

Figure 1 Tumor necrosis factor-a stimulated porcine splenocytes activates human T cells

The activation was inhibited by addition of antihuman B7 antibodies, which cross-reacts with pig CD80. hu1F1, cross-reactive antihCD80; mu4B2, non-crossreactive antihCD80; CTLA4-Ig; 9.3-Fab fragment, antihCD28.

Tadaki, D. K., Williams, A., Lee, K. P., Kirk, A. D., & Harlan, D. M. (2003). Porcine CD80: cloning, characterization, and evidence for its role in direct human T‐cell activation. Xenotransplantation, 10(3), 252-258.

ELISA

Figure 2 a graph depicting the results of a competitive binding assay.

Figure 2 a graph depicting the results of a competitive binding assay.

The graph depicts the results of a competitive binding assay of murine or humanized anti-human B7-1 mAbs to CHO transfected with rhB7-1 (CHO/hB7-1). Increasing concentrations of unlabeled competitor antibodies were incubated with CHO/hB7-1 cells in the presence of radiolabeled tracer humanized 1F1, and the ratio of bound/ free antibody was determined.

Vasquez, M., Carreno, B., Celniker, A. C., Collins, M., Goldman, S., Gray, G. S., ... & Veldman, G. M. (2005). U.S. Patent No. 6, 913, 747. Washington, DC: U.S. Patent and Trademark Office.


Specifications

  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Humanized IgG4, κ
  • Specificity
  • Human CD80
  • Species Reactivity
  • Human
  • Clone
  • hu1F1
  • Applications
  • ELISA, FuncS
  • Related Disease
  • Autoimmune Diseases, Transplant Rejection, Inflammatory Disorders, Infectious Diseases

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody hu1F1 has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1; h1F1; hu1F1; humanized 1F1

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone hu1F1"

See other products for "CD80"

Chimeric Antibody

CAT Product Name Application Type
TAB-215 Anti-Human CD80 Recombinant Antibody (Galiximab) WB, ELISA, FC, IP, FuncS, IF, Neut IgG1 - lambda

Immunotoxin

CAT Product Name Application Type
AGTO-L001G anti-CD80 immunotoxin 6G7 (IgG)-Gel Cytotoxicity assay, Functional assay
AGTO-L001S anti-CD80 immunotoxin 6G7 (IgG)-Sap Cytotoxicity assay, Functional assay
AGTO-L001O anti-CD80 immunotoxin 6G7 (IgG)-Bouganin Cytotoxicity assay, Functional assay

Fc Glycosylation

CAT Product Name Application Type
Gly-159LC Recombinant Anti-Human CD80 Antibody (Fc glycosylation/Non fucosylated) ELISA Chimeric antibody (primate/human)

Low- or Non-fucosylated Oligosaccharide

CAT Product Name Application Type
Gly-159LC-1 Recombinant Anti-Human CD80 Antibody (Fc glycosylation/Non fucosylated) ELISA Chimeric antibody (primate/human)

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0066MZ Chicken Anti-BB1 Polyclonal IgY WB Chicken antibody

Neutralizing Antibody

Blocking Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0583 Hi-Affi™ Rabbit Anti-CD80 Recombinant Antibody (clone DS583AB) WB, IHC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-215 Afuco™ Anti-CD80 ADCC Recombinant Antibody (Galiximab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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