Human Anti-CD80 Recombinant Antibody (clone hu1F1)

Name: Human Anti-CD80 Recombinant Antibody (clone hu1F1)
SKU: HPAB-0063-YJ
Rating: 5 (1 reviews)

CAT#: HPAB-0063-YJ

This product is a recombinant humanized antibody that recognizes human B7-1. Mab hu1F1 functions in treatments for various autoimmune diseases, transplant rejection, inflammatory disorders, and infectious diseases.

Published Data Gene Expression

ELISA

Figure 2 a graph depicting the results of a competitive binding assay.

The graph depicts the results of a competitive binding assay of murine or humanized anti-human B7-1 mAbs to CHO transfected with rhB7-1 (CHO/hB7-1). Increasing concentrations of unlabeled competitor antibodies were incubated with CHO/hB7-1 cells in the presence of radiolabeled tracer humanized 1F1, and the ratio of bound/ free antibody was determined.

Vasquez, M., Carreno, B., Celniker, A. C., Collins, M., Goldman, S., Gray, G. S., ... & Veldman, G. M. (2005). U.S. Patent No. 6, 913, 747. Washington, DC: U.S. Patent and Trademark Office.

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Normal Tissue Figure 1 Bone marrow

Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25368/56116_B_6_4.jpg

Normal Tissue Figure 2 Lymph node

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25368/56076_A_9_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, Karpas-707, U-266/70)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000121594-CD80

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Specifications

Host Species
Human

Derivation
Humanized

Type
Humanized IgG4, κ

Specificity
Human CD80

Species Reactivity
Human

Clone
hu1F1

Applications
ELISA, FuncS

Related Disease
Autoimmune Diseases, Transplant Rejection, Inflammatory Disorders, Infectious Diseases

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody hu1F1 has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

Alternative Names
CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1; h1F1; hu1F1; humanized 1F1

Gene ID
941

UniProt ID
P33681

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Related Diseases

Rheumatoid Arthritis

Systemic Lupus Erythematosus

Allograft Rejection

Autoimmune Thyroid Disease

Graft-versus-Host Disease

Maturity Onset Diabetes of the Young

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0063-YJ.pdf

MSDS

MSDS - HPAB-0063-YJ.pdf

COA

Certificate of Analysis Lookup

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