Human Anti-EPCAM Recombinant Antibody (clone HuMAB UBS-54) (CAT#: HPAB-0163CQ)

Figure 1 Localization of the fully human anti-EpCAM-directed UBS54 Ab applied i.p. in EpCAM-transgenic FVB/N-C57BL/6 hybrid mice.

UBS54 localization was determined immunohistochemically 48 h after injection by staining with an anti-human IgG peroxidase conjugate. UBS54 localized on the EpCAM-expressing epithelial cell lining of the kidney (a), uterus (c), bladder (e), pancreas (f), colon (h), lung (j), liver (k), and salivary glands (o), whereas no UBS54 was observed in EpCAM-negative tissues, such as, for instance, heart (m). No specific staining was observed after incubation of similar EpCAM-expressing tissue sections obtained from transgenic mice injected with PBS: kidney (b), uterus (d), pancreas (g), colon (i), liver (l), and on slides of EpCAM-negative heart tissue (n).

Kosterink, J. G., McLaughlin, P. M., Lub-de Hooge, M. N., Hendrikse, H. H., van Zanten, J., van Garderen, E.,... & de Leij, L. F. (2007). Biodistribution studies of epithelial cell adhesion molecule (EpCAM)-directed monoclonal antibodies in the EpCAM-transgenic mouse tumor model. The Journal of Immunology, 179(2), 1362-1368.

Figure 2 Biodistribution of ̶¹²⁵I-labeled mAbs in most relevant tissues and organs in EpCAM-transgenic FVB/N-V57BL/6 hybrid mice and in nontransgenic FVB/N-C57BL/6 hybrid mice 48 h after injection.

Biodistribution is expressed as tissue-to-blood ratio per gram tissue.

Kosterink, J. G., McLaughlin, P. M., Lub-de Hooge, M. N., Hendrikse, H. H., van Zanten, J., van Garderen, E.,... & de Leij, L. F. (2007). Biodistribution studies of epithelial cell adhesion molecule (EpCAM)-directed monoclonal antibodies in the EpCAM-transgenic mouse tumor model. The Journal of Immunology, 179(2), 1362-1368.

Figure 1 Anti-EPCAM Antibody (HPAB-0163CQ) in ELISA

ELISA analysis of HPAB-0163CQ was performed by coating with Recombinant Human EpCAM Protein. Then blocked with BSA, and incubated with Anti-EPCAM Antibody (HPAB-0163CQ). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-EPCAM Antibody (HPAB-0163CQ) in WB

Western blot analysis of HPAB-0163CQ was performed by loading Recombinant Human EpCAM Protein. Lane 1: Non-Reducing Antigen (1ug). Lane 2: Reducing Antigen (1ug).