Recombinant Rabbit Anti-FAS Antibody (clone R02-8I7)
CAT#: VS3-FY535
This product is a recombinant rabbit antibody that recognizes FAS. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R02-8I7 is specific for human FAS. The antigen is human fas synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/546_F3_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
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WB
Figure 1. Recombinant Rabbit Anti-FAS Antibody (clone R02-8I7) in WB.
Western Blot analysis of Fas in Hela, Jurkat lysates using Fas Antibody.
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Specifications
- Immunogen
- Human Fas Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FAS
- Species Reactivity
- Human
- Clone
- R02-8I7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 38 kDa; Observed MW: 40-50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Fas Cell Surface Death Receptor; Tumor Necrosis Factor Receptor Superfamily, Member 6; Fas (TNF Receptor Superfamily, Member 6); Apoptosis-Mediating Surface Antigen FAS; TNF Receptor Superfamily Member 6; FASLG Receptor; CD95 Antigen; TNFRSF6; APT1; FAS1; Mutant Tumor Necrosis Receptor Superfamily Member 6
- Gene ID
- 355
- UniProt ID
- P25445
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions.
Palmitoylated (PubMed:25301068).
Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane (PubMed:25301068).
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Protein Refseq
- NP_000034.1; NP_001307548.1; NP_690610.1
- Function
- Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
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Related Signaling Pathways
TNF Signaling Pathway
Related Diseases
Allograft Rejection
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Graft-versus-Host Disease
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-094 | Human Anti-FAS Recombinant Antibody (clone HFE7A); Fab Fragment | ELISA, WB, IF, FuncS | Human Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); scFv Fragment | WB, IF, FuncS | Human scFv |
| HPAB-0450-YJ-S(P) | Rabbit Anti-FAS Recombinant Antibody; scFv Fragment (HPAB-0450-YJ-S(P)) | ELISA, FC | Rabbit scFv |
| HPAB-0451-YJ-S(P) | Rabbit Anti-FAS Recombinant Antibody; scFv Fragment (HPAB-0451-YJ-S(P)) | ELISA, FC | Rabbit scFv |
| HPAB-1575WJ-S(P) | Mouse Anti-FAS Recombinant Antibody; scFv Fragment (clone F919-9-18) | ELISA, WB | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-029WM-S(P) | Anti-Human FAS Recombinant Antibody scFv Fragment (F45D9-γ1) | IHC, ELISA, FC | Human Antibody |
| TAB-029WM-F(E) | Anti-Human FAS Recombinant Antibody Fab Fragment (F45D9-γ1) | IHC, ELISA, FC | Human Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0201MZ | Chicken Anti-FAS (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0202MZ | Chicken Anti-FAS (ab2) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-767CQ | Mouse Anti-FAS Recombinant Antibody (clone CBL933) | Neut, FC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-768CQ | Mouse Anti-FAS Recombinant Antibody (clone ZB4) | Block, FC, IHC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1252 | Hi-Affi™ Rabbit Anti-FAS Recombinant Antibody (clone DS1252AB) | WB, ICC | Rabbit IgG |
| MOR-1253 | Hi-Affi™ Rabbit Anti-Fas Recombinant Antibody (clone DS1253AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-103LC | Mouse Anti-FAS Recombinant Antibody (clone CH11) | FC, ICC, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FN-213CQ | Mouse Anti-FAS Recombinant Antibody (clone EOS9.1) | FC, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC127 | Human Anti-FAS Recombinant Antibody Hexamer (VS-0924-YC127), CDC Enhanced | IHC, FC, ADCC, CDC | Antibody hexamer |
| VS-0924-YC128 | Mouse Anti-FAS Recombinant Antibody Hexamer (VS-0924-YC128), CDC Enhanced | ELISA, WB, CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC348 | Recombinant Anti-FAS Vesicular Antibody, EV Displayed (VS-0425-YC348) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2448 | Anti-FAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY2449 | Anti-Human FAS Immunohistochemistry Kit | IHC |
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