Mouse Anti-CD19 Recombinant Antibody (VS-1125-XY2)

Name: Mouse Anti-CD19 Recombinant Antibody (VS-1125-XY2)
SKU: VS-1125-XY2
Rating: 5 (1 reviews)

CAT#: VS-1125-XY2

Specially crafted for cell therapy development and companion diagnostic applications, this CD19 recombinant antibody exhibits outstanding sensitivity in detecting low - abundance CD19 expression. It is fully compatible with ELISA for quantitative analysis, IHC for tissue localization, and FC for cell sorting, making it suitable for investigating diverse biological samples ranging from fresh primary cells to frozen tissue sections. Additionally, it has undergone rigorous testing to ensure minimal cross - reactivity, guaranteeing accurate target identification.

Gene Expression

Normal Tissue Figure 1 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg

Normal Tissue Figure 2 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg

Normal Tissue Figure 3 Appendix

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19

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Specifications

Host Animal
Mouse

Applications
IHC, ICC, IF, FC

Specificity
CD19

Target
CD19

Species Reactivity
Human

Type
Mouse IgG1, kappa

Purity
>95%, determined by SDS-PAGE

Purification
Affinity chromatography

Format
Liquid

Buffer
PBS, pH 7.2

Preservative
0.09% sodium azide

Storage
Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Target

Introduction
CD19 is a transmembrane glycoprotein expressed primarily on B lymphocytes, spanning pre-B cells to mature B cells (excluding plasma cells). It functions as a key co-receptor in B cell activation, signaling, and proliferation by associating with CD21, CD81, and Leu-13. As a hallmark of B cell lineage, it is widely targeted in hematologic malignancies like B-cell acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Therapies such as CAR-T cells (e.g., tisagenlecleucel) and monoclonal antibodies leverage CD19's restricted expression to eliminate malignant B cells, offering transformative treatment options for refractory cases.

Alternative Names
B4, CVID3

Gene ID
930

UniProt ID
P15391

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

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Datasheet

Datasheet - VS-1125-XY2.pdf

MSDS

MSDS - VS-1125-XY2.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Secondary Antibody

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Isotype Control

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