Anti-EpCAM Immunocytokine, IgG (KS-1/4)-IL12-IL2 (ACFP-SH179)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EpCAM IgG to IL12 and IL2. It was expressed in CHO and purified with affinity chromatography. The Immunocytokines retains the ability to bind the EpCAM as well as the biological activity of IL12 and IL2. KS-IL12-IL2 caused a profound inhibition of tumor growth, the multiple-cytokine antibody fusion protein can be used to treat cancer in an immunosuppressed animal. In the context of bi-functional fusion proteins, hetero-dimeric IL-12 could be expressed either in a single-chain form, or maintained as a heterodimer in which the p40 subunit was fused to IL-2. These IL-12/IL-2 bi-functional fusion proteins were shown to induce extremely high levels of interferon-gamma (IFN-γ), similar to the synergy normally seen with the combined application of the individual cytokines. This Immunocytokines was designed for treating solid tumor.
Specifications
- Target
- EpCAM
- Molecule Class
- IgG1-IL12-IL2
- Species Reactivity
- Human
- Host
- Mouse
- Expression Host
- CHO
- Clone
- KS-1/4
- Related Disease
- Solid tumor
- Cytokines 1
- IL12
- Cytokines 1 Description
- Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.
- Cytokine 1 Species
- Human
- Cytokines 2
- IL2
- Cytokines 2 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 2 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2
- Entrez Gene ID
- 4072
- UniProt ID
- P16422
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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