Anti-Ley Immunocytokine, TNFα-scFv (B1) (ACFP-SH165)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-Ley scFv to TNFα. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the Ley as well as the biological activity of TNFα. The rationale for fusing the scFv to the COOH terminus of TNF was to diminish the binding of the fusion protein to TNF receptors because the COOH terminus of TNF is involved in binding, and thus to partially inactivate (detoxify) the molecule. This molecule binds to LeY antigen on cancer cells with the same affinity as B1 (scFv) and B1 (scFv) immunotoxins but with significantly lower affinity to the TNF receptor compared to the TNF trimer. This immunocytokine was designed for treating Ley-expressing TNF-sensitive cancer.
Specifications
- Target
- Ley
- Molecule Class
- TNFα-scFv
- Species Reactivity
- Human
- Host
- Mouse
- Expression Host
- CHO
- Clone
- B1
- Related Disease
- Ley-expressing TNF-sensitive cancer
- Cytokines 1
- TNFα
- Cytokines 1 Description
- Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD). While still controversial, studies of depression and IBD are currently being linked to TNF levels. Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- Lewis Y; LeY
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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