Anti-Human TCR Recombinant Antibody (UC3-10A6) (CAT#: TAB-562CT)

Figure 1 Vγ4 T cells have a negative impact on skin wound healing.

Age- and sex-matched C57BL/6 wild-type mice were intraperitoneally injected with 200 μg/mouse Vγ4 T-cell depletion (Vγ4D) Ab UC3 on day 3 before wound excision. Full-thickness wounds were then generated using a sterile 6 mm punch tool on day 0. Wound-closure kinetics was measured over time in wound model without contraction.

Li, Y., Wang, Y., Zhou, L., Liu, M., Liang, G., Yan, R., ... & Huang, Y. (2018). Vγ4 T cells inhibit the pro-healing functions of dendritic epidermal T cells to delay skin wound closure through IL-17A. Frontiers in Immunology, 9, 240.

Figure 2 Vγ4 T cells inhibited epidermal IGF-1 production to delay skin wound repair.

Full-thickness wounds were generated in Vγ4 T-cell depletion (Vγ4D) and control mice. On day 3, 5, and 7 post-wounding, each group of epidermis tissues at wound margins was collected from at least three mice for further analysis of IGF-1 production by means of WB.

Li, Y., Wang, Y., Zhou, L., Liu, M., Liang, G., Yan, R., ... & Huang, Y. (2018). Vγ4 T cells inhibit the pro-healing functions of dendritic epidermal T cells to delay skin wound closure through IL-17A. Frontiers in Immunology, 9, 240.

Figure 3 Vγ4 T cells inhibited epidermal IGF-1 production to delay skin wound repair.

Full-thickness wounds were generated in Vγ4 T-cell depletion (Vγ4D) and control mice. On day 3, 5, and 7 post-wounding, each group of epidermis tissues at wound margins was collected from at least three mice for further analysis of IGF-1 production by means of IHC.

Li, Y., Wang, Y., Zhou, L., Liu, M., Liang, G., Yan, R., ... & Huang, Y. (2018). Vγ4 T cells inhibit the pro-healing functions of dendritic epidermal T cells to delay skin wound closure through IL-17A. Frontiers in Immunology, 9, 240.

Figure 4 IGF-1 production by dendritic epidermal T cells (DETCs) around wound area was enhanced in Vγ4 T cell depletion mice.

The percentages of Vγ5 T cells in epidermis at wound margin of Vγ4 T-cell depletion (Vγ4D) and control mice were detected by FACS on days 0 after wounding.

Li, Y., Wang, Y., Zhou, L., Liu, M., Liang, G., Yan, R., ... & Huang, Y. (2018). Vγ4 T cells inhibit the pro-healing functions of dendritic epidermal T cells to delay skin wound closure through IL-17A. Frontiers in Immunology, 9, 240.

Figure 5 Age- and sex-matched TCRδ-GFP mice were intraperitoneally injected with Vγ4D Ab UC3 or isotype control Ab (Control) on day −3.

The morphology of DETCs around the wounds in Vγ4D and wild-type (WT) control mice were analyzed by confocal microscopy.

Li, Y., Wang, Y., Zhou, L., Liu, M., Liang, G., Yan, R., ... & Huang, Y. (2018). Vγ4 T cells inhibit the pro-healing functions of dendritic epidermal T cells to delay skin wound closure through IL-17A. Frontiers in Immunology, 9, 240.