Anti-Human TCR Recombinant Antibody (T10B9) (CAT#: TAB-548CT)

Recombinant monoclonal antibody specifically binds to T cell receptor. This antibody can be potentially used in treating T-cell malignancies.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
FC

Figure 1 SPV-T3b pretreatment enables to distinguish tetramer-binding to the TCR/CD3 complex from TCR unrelated binding among PBMC.

Figure 1 SPV-T3b pretreatment enables to distinguish tetramer-binding to the TCR/CD3 complex from TCR unrelated binding among PBMC.

PBMC from healthyHLA-A2þdonors were incubated with HLA-A2/flu tetramers (horizontal axis), after preincubation with anti-TCR mAb T10B9, followed byGAM-Ig.

Weder, P., Schumacher, T. N., Spits, H., & Luiten, R. M. (2012). Testing for HLA/peptide tetramer-binding to the T cell receptor complex on human T lymphocytes. Results in immunology, 2, 88-96.

FC

Figure 2 Flow cytometric analysis of lymphoid cells in peripheral blood after administration of T10B9.

Figure 2 Flow cytometric analysis of lymphoid cells in peripheral blood after administration of T10B9.

There is persistent modulation of the CD3/TCR αβ region in these phenotypically altered T cells for 10 days while the mABs are being administered with repopulation over 15-40 days.

Waid, T. H., Thompson, J. S., Siemionow, M., & Brown, S. A. (2009). T10B9 monoclonal antibody: A short-acting nonstimulating monoclonal antibody that spares γδ T-cells and treats and prevents cellular rejection. Drug design, development and therapy, 3, 205.

Cyt

Figure 3 Cytokine release in vivo in patients undergoing treatment for acute cellular rejection with T10B9 or OKT3.

Figure 3 Cytokine release in vivo in patients undergoing treatment for acute cellular rejection with T10B9 or OKT3.

T10B9 is nonmitogenic and causes minimalcytokine (TNFα and IFNγ) release producingfewer and milder untoward effects.

Waid, T. H., Thompson, J. S., Siemionow, M., & Brown, S. A. (2009). T10B9 monoclonal antibody: A short-acting nonstimulating monoclonal antibody that spares γδ T-cells and treats and prevents cellular rejection. Drug design, development and therapy, 3, 205.

Inhib

Figure 4 Inhibition of tetramer-binding to T cells by preincubation with anti-CD3 and anti-TCR antibodies.

Figure 4 Inhibition of tetramer-binding to T cells by preincubation with anti-CD3 and anti-TCR antibodies.

CTL INFA24 was preincubated with 3-fold increasingconcentrations ranging from 0.07–50 μg/ml of anti-CD3 antibodies, SPV-T3b (○), OKT3 (■), and anti-TCR antibodies WT31 (△) and T10B9 (●) or control IgG (X), followedby incubations with GAM-Ig and HLA-A2/flu tetramer. Left panel: binding intensity (MFI) of anti-CD3 or anti-TCR antibodies or control IgG to CTLINFA24 T cells.

Weder, P., Schumacher, T. N., Spits, H., & Luiten, R. M. (2012). Testing for HLA/peptide tetramer-binding to the T cell receptor complex on human T lymphocytes. Results in immunology, 2, 88-96.


Specifications

  • Host Species
  • Humanized
  • Type
  • Humanized antibody
  • Specificity
  • Human
  • Species Reactivity
  • Human
  • Clone
  • T10B9
  • Applications
  • FC, Cyt, Inhib
  • Related Disease
  • Autoimmune Disease, T-cell malignancies

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry, Cytotoxicity and Inhibition assay.

Target

  • Alternative Names
  • TCR; T cell receptor; TCRA; TCRB

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone T10B9"

See other products for "TCR"

Recombinant Antibody

Mouse Antibody

CAT Product Name Application Type
TAB-595CL Mouse Anti-TCR Recombinant Antibody (TAB-595CL) FuncS Mouse IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-248 Afuco™ Anti-TCR ADCC Recombinant Antibody (Maslimomab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for TAB-548CT. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare