Anti-Human TCR Recombinant Antibody (T10B9) (CAT#: TAB-548CT)

Figure 1 SPV-T3b pretreatment enables to distinguish tetramer-binding to the TCR/CD3 complex from TCR unrelated binding among PBMC.

PBMC from healthyHLA-A2þdonors were incubated with HLA-A2/flu tetramers (horizontal axis), after preincubation with anti-TCR mAb T10B9, followed byGAM-Ig.

Weder, P., Schumacher, T. N., Spits, H., & Luiten, R. M. (2012). Testing for HLA/peptide tetramer-binding to the T cell receptor complex on human T lymphocytes. Results in immunology, 2, 88-96.

Figure 2 Flow cytometric analysis of lymphoid cells in peripheral blood after administration of T10B9.

There is persistent modulation of the CD3/TCR αβ region in these phenotypically altered T cells for 10 days while the mABs are being administered with repopulation over 15-40 days.

Waid, T. H., Thompson, J. S., Siemionow, M., & Brown, S. A. (2009). T10B9 monoclonal antibody: A short-acting nonstimulating monoclonal antibody that spares γδ T-cells and treats and prevents cellular rejection. Drug design, development and therapy, 3, 205.

Figure 3 Cytokine release in vivo in patients undergoing treatment for acute cellular rejection with T10B9 or OKT3.

T10B9 is nonmitogenic and causes minimalcytokine (TNFα and IFNγ) release producingfewer and milder untoward effects.

Waid, T. H., Thompson, J. S., Siemionow, M., & Brown, S. A. (2009). T10B9 monoclonal antibody: A short-acting nonstimulating monoclonal antibody that spares γδ T-cells and treats and prevents cellular rejection. Drug design, development and therapy, 3, 205.

Figure 4 Inhibition of tetramer-binding to T cells by preincubation with anti-CD3 and anti-TCR antibodies.

CTL INFA24 was preincubated with 3-fold increasingconcentrations ranging from 0.07–50 μg/ml of anti-CD3 antibodies, SPV-T3b (○), OKT3 (■), and anti-TCR antibodies WT31 (△) and T10B9 (●) or control IgG (X), followedby incubations with GAM-Ig and HLA-A2/flu tetramer. Left panel: binding intensity (MFI) of anti-CD3 or anti-TCR antibodies or control IgG to CTLINFA24 T cells.

Weder, P., Schumacher, T. N., Spits, H., & Luiten, R. M. (2012). Testing for HLA/peptide tetramer-binding to the T cell receptor complex on human T lymphocytes. Results in immunology, 2, 88-96.