The CBD that bound Alkaline phosphatase was isolated from the library using Phage display techonology. CBD is a member of the knottin family which is a kind of small, disulphide-bonded proteins that bind with high specificity to their target molecules. After affinity maturation, CBD variants were identified having affinities (KD=10 μM) for Alkaline phosphatase as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specifications
- Scaffold Name
- CBD
- Origin
- Fungal enzyme cellobiohydrolase I
- Core Structure
- 3β-sheet (36)
- Variable Regions
- 11 aa on loops or 7 aa on flat surface
- Target
- Alkaline phosphatase
- Species Reactivity
- Bovine
- Expression Host
- E. coli
- Affinity Constant
- 10 μM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fungal enzyme cellobiohydrolase I; CBD; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
- Entrez Gene ID
- 280993
- UniProt ID
- P19111.2
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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