The CBM4-2 that bound calcium ions was isolated from the library using Phage display techonology. The CBM4-2, an 18-kDa protein derived from the xylanase (Xyn 10A) of Rhodothermus marinus, has a β-sandwich structure formed by 11 strands and contains no disulfide bonds. It is a well characterized module with defined substrate affinity and specificity. This module is thermostable and suitable for evolution of new binding properties. After affinity maturation, CBM4-2 variants were identified having affinities for calcium ions as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Specifications

Scaffold Name
CBM4-2

Origin
Xylanase 10A (Xyn10A)

Core Structure
β-sandwich

Variable Regions
12 residues in carbohydrate binding site

Target
Calcium ions

Expression Host
E. coli

Applications
ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study

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CBM4-2 scaffold protein anti-Calcium ions (CAT#: SCM-L052)

Specifications

Target

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