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Anti-CD33 (HuM195)-AcBut-Calicheamicin ADC (CAT#: ADC-056LCT)

This ADC product is composed of an anti-CD33 antibody (clone HuM195) conjuagated via a AcBut linker to Calicheamicin (HuM195-AcBut-Calicheamicin). It has demonstrated a response in AML treatment by a MOA (Mechanism of Action) of Calicheamicins target DNA and cause strand scission. Calicheamicins bind with DNA in the minor groove.


Specific Inquiry
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1186_A4_3_selected.jpg
Figure 2 IHC staining of human lymph node
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows membranous and cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35832/113039_A_9_8_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11442/26430_A_6_8_selected.jpg
Figure 4 IF staining of human cell line RH-30
Figure 4 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1413_G6_1_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64807/1200_A4_2_red_green.jpg
Normal Tissue
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_5.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_A_9_8.jpg
Figure 8 Spleen
Figure 8 Spleen
(Cells in red pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79996/170702_B_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
Figure 9 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, RPMI-8226, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105383-CD33

Specifications

  • Antibody Overview
  • Humanized version of the Antibody M195 directed against CD33
  • Clone
  • HuM195
  • Antibody Isotype
  • IgG1
  • Linker
  • AcBut
  • Linker Class/Description
  • Acid-labile linkers, belonging to chemically cleavable linkers, are designed based on a pH-dependent release mechanism and remain intact during systemic circulation in the blood's neutral pH environment (pH 7.3-7.5) but to undergo hydrolysis and release drug once the ADC is internalized into mildly acidic endosomal (pH 5.0-6.5) and lysosomal (pH 4.5-5.0) compartments of the cell.
  • Drug
  • Calicheamicin
  • Drug Class/Description
  • Calicheamicin, produced by prokaryotic microorganisms, are a group of DNA cleaving agents with similar structure that binds in the minor grove of DNA in a relatively sequence-specific manner.

Target

  • Introduction
  • CD33 or Siglec-3 is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. It binds sialic acids, therefore is a member of the SIGLEC family of lectins.
  • Alternative Names
  • CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3;

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NAB-321-sdAb Recombinant Anti-human CD33 VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-013 Humanized Anti-CD33 Recombinant Antibody (clone Gemtuzumab) IF, IP, Neut, FuncS, ELISA, FC, WB Humanized (from mouse) IgG4, κ
TAB-H37 Anti-Human CD33 Recombinant Antibody (Gemtuzumab Ozogamicin) ELISA, FC, IP, FuncS, IF, Neut, IHC IgG
TAB-756 Anti-CD33 Recombinant Antibody (Lintuzumab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
VS3-WK1523 Human Anti-CD33 Recombinant Antibody (clone CDP-771) FC Humanized IgG4

Immunotoxin

CAT Product Name Application Type
AGTO-G008E Anti-CD33 immunotoxin (scFv)-PE Cytotoxicity assay, Function study
AGTO-G008G Anti-CD33 immunotoxin (scFv)-Gel Cytotoxicity assay, Function study
AGTO-L050E anti-CD33 immunotoxin M195 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L050G anti-CD33 immunotoxin M195 (IgG)-Gel Cytotoxicity assay, Functional assay

Mouse Antibody

CAT Product Name Application Type
TAB-119LC Mouse Anti-CD33 Recombinant Antibody (TAB-119LC) ELISA, IF, RIA Mouse IgG
TAB-120LC Mouse Anti-CD33 Recombinant Antibody (TAB-120LC) ELISA, Cyt Mouse IgG
TAB-121LC Mouse Anti-CD33 Recombinant Antibody (TAB-121LC) ELISA Mouse IgG
TAB-122LC Mouse Anti-CD33 Recombinant Antibody (TAB-122LC) ELISA, Cyt Mouse IgG
TAB-124LC Mouse Anti-CD33 Recombinant Antibody (TAB-124LC) ELISA, FC Mouse IgG

Human Antibody

CAT Product Name Application Type
TAB-123LC Anti-Human CD33 Recombinant Antibody FC Human antibody
TAB-146LC Human Anti-CD33 Recombinant Antibody (TAB-146LC) ELISA Human IgG
TAB-123LC-S(P) Anti-Human CD33 Recombinant Antibody scFv Fragment FC Human antibody
TAB-146LC-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (TAB-146LC-S(P)) ELISA Human scFv
TAB-123LC-F(E) Anti-Human CD33 Recombinant Antibody Fab Fragment FC Human antibody

Fab Glycosylation

CAT Product Name Application Type
Gly-013LC Recombinant Anti-Human CD33 Antibody (Fab glycosylation) ELISA Humanized antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-341CQ Mouse Anti-CD33 Recombinant Antibody (clone WM53) FC, IHC-Fr, ICC, IP, WB, Block Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0553 Hi-Affi™ Rabbit Anti-CD33 Recombinant Antibody (clone DS553AB) WB, ICC, IP Rabbit IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-471CQ Anti-Human CD33 Recombinant Antibody (Vadastuximab) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

Recombinant Antibody

CAT Product Name Application Type
HPAB-0087-FY Human Anti-CD33 Recombinant Antibody (HPAB-0087-FY) ELISA, ADCC Human IgG
HPAB-M0471-YC Human Anti-CD33 Recombinant Antibody (HPAB-M0471-YC) FuncS Humanized IgG
HPAB-1929LY Human Anti-CD33 Recombinant Antibody (HPAB-1929LY) FC Humanized IgG
HPAB-1930LY Human Anti-CD33 Recombinant Antibody (HPAB-1930LY) FC Humanized IgG
HPAB-1931LY Human Anti-CD33 Recombinant Antibody (HPAB-1931LY) FC Humanized IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-471CQ Afuco™ Anti-CD33 ADCC Recombinant Antibody (Vadastuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-756 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Lintuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-013 Afuco™ Anti-CD33 ADCC Recombinant Antibody (Gemtuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-1973LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1973LY-F(E)) FC Humanized Fab
HPAB-1974LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1974LY-F(E)) FC Humanized Fab
HPAB-1975LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1975LY-F(E)) FC Humanized Fab
HPAB-1976LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1976LY-F(E)) FC Humanized Fab
HPAB-1977LY-F(E) Human Anti-CD33 Recombinant Antibody; Fab Fragment (HPAB-1977LY-F(E)) FC Humanized Fab

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-CN0509 PE-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-CN0510 APC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-CN0511 FITC-A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-CN0512 A*02:01/Human CD33 (YLISGDSPV) MHC Tetramer FCM, IHC
MHC-YF358 A*02:01/Human CD33 65-73 (1Y2L) (YLISGDSPV) MHC Pentamer FCM

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0739-FY-F(E) Mouse Anti-CD33 Recombinant Antibody (clone KM4072); scFv Fragment ELISA Mouse scFv
HPAB-0740-FY-F(E) Mouse Anti-CD33 Recombinant Antibody (clone KM4073); scFv Fragment ELISA Mouse scFv
HPAB-0741-FY-F(E) Mouse Anti-CD33 Recombinant Antibody (clone KM4074); scFv Fragment ELISA Mouse scFv
HPAB-0742-FY-F(E) Mouse Anti-CD33 Recombinant Antibody (clone KM4075); scFv Fragment ELISA Mouse scFv
HPAB-0374-FY-S(P) Human Anti-CD33 Recombinant Antibody; scFv Fragment (HPAB-0374-FY-S(P)) FC, ELISA Human scFv

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