What controls should I use in my experiments?

Use an appropriate isotype control and unstained cell samples to set baselines and ensure specificity in your flow cytometry experiments.

Why am I seeing high background staining?

High background staining could be due to non-specific binding or insufficient washing. Ensure you follow stringent washing protocols and use appropriate blocking agents.

How do I determine the optimal dilution for my samples?

It's best to perform a titration experiment to determine the optimal dilution. Start with the recommended concentration and adjust based on your specific assay conditions.

A*02:01/Human MAGE-A10 (GLYDGMEHL) MHC Monomer (CAT#: MHC-YF237)

This product is a MHC monomer of biotinylated peptide/MHC complex composed MAGE-A10-derived peptide of GLYDGMEHL sequence and A*02:01 molecule. The pMHC monomer recognizes human CD8 T cells and can be used in the MHC tetramer analysis of individual antigen-specific T cells.

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Custom Production

MHC tetramer custom production provides a vital tool for researchers seeking to understand and manipulate T-cell immunity with exceptional precision. Please specify your requirements, including peptide sequence, MHC allele, and desired fluorophore etc. We will respond as soon as possible with a tailored solution.

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Antigen Molecule:

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Disclaimer: Please note that the MHC reagents we offered have been produced with strict quality control (including gene sequencing, affinity purification, SDS-PAGE analysis, and verification of biotinylation), but many have not been confirmed to stain or activate specific T cells.

We require custom production

Gene Expression
Datasheet
MSDS
COA

Subcellular Location

Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70780/1696_F2_1_selected.jpg

Normal Tissue

Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3333/ihc_selected.jpg

Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3333/9906_B_7_5.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3333/9906_A_8_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3333/9906_A_4_6.jpg

Figure 6 Placenta
(Decidual cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3333/9906_A_3_7.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enriched (GAMG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124260-MAGEA10

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Allele
A*02:01

Class
Class I

MHC Species
Human

Antigen
MAGE-A10

Antigen Species
Human

Peptide
GLYDGMEHL

Conjugate
Biotin

Application
MHC Multimer

Target

Antigen Introduction
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.

Alternative Names
CT1.10; MAGE10

Gene ID
4109

UniProt ID
P43363

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MHC Tetramers for Cancer Antibody Magnetic Beads

CAT	Product Name	Application
MHC-CN1013	PE-A*02:01/Human MAGEA10 (GLYDGMEHL) MHC Tetramer	FCM, IHC
MHC-CN1014	APC-A*02:01/Human MAGEA10 (GLYDGMEHL) MHC Tetramer	FCM, IHC
MHC-CN1015	FITC-A*02:01/Human MAGEA10 (GLYDGMEHL) MHC Tetramer	FCM, IHC
MHC-CN1016	A*02:01/Human MAGEA10 (GLYDGMEHL) MHC Tetramer	FCM, IHC

CAT	Product Name	Application	Type
VS-0724-YC250	AbPlus™ Anti-MAGEA10 Magnetic Beads (VS-0724-YC250)	IP, Protein Purification

Customer Reviews and Q&As

Customer Review Q&As

Easy to Use

The protocol provided was straightforward and easy to follow. I had no issues staining my cells and the results were consistent with my expectations.

Reproducible Results

I've been using this MHC Pentamer for several projects now, and the results have been reliable and reproducible every time. I highly recommend it for immuno-monitoring studies.

Excellent Customer Support

The customer service team was extremely helpful in answering all my questions regarding the product. They guided me through optimization steps for my specific assay.

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