Mouse Anti-CD19 Recombinant Antibody (clone 25C1); scFv Fragment
CAT#: FAMAB-0071WJ-S(P)
This product is a mouse monoclonal antibody scFv fragment that recognizes the CD19 antigen found on normal and malignant B cells, but not on stem cells. This antibody scFv fragment has the potential for relatively selective treatment of cancer diseases.
Gene Expression
Normal Tissue
Figure 1 Colon
Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg
Normal Tissue
Figure 2 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg
Normal Tissue
Figure 3 Appendix
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Human scFv
- Specificity
- Human CD19
- Species Reactivity
- Human
- Clone
- 25C1
- Applications
- WB, FC
- Related Disease
- Cancers
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
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Excellent Stability
The storage stability of this antibody fragment is remarkable. Even after several months at -20°C, it still performs as well as the first day, providing robust and reliable data.
High Specificity
The high specificity of this scFv Fragment for CD19 makes my immunoassays much more accurate. There's minimal nonspecific binding, which has improved the quality of my results.
Effective in Flow Cytometry
Perfect for flow cytometry applications! The Mouse Anti-CD19 Recombinant Antibody (clone 25C1); scFv Fragment works exceptionally well in detecting CD19-positive cells, and I've seen great results every time.
Q&As
-
When utilized in flow cytometry applications, how specific is the Mouse Anti-CD19 Recombinant Antibody (clone 25C1); scFv Fragment?
A: The Mouse Anti-CD19 Recombinant Antibody (clone 25C1); scFv Fragment shows high specificity for CD19-positive cells when used in flow cytometry. It effectively distinguishes CD19-expressing cells from other cell types, providing reliable and precise data.
-
Does this antibody fragment attach itself to a particular CD19 molecule epitope?
A: Yes, it targets a specific epitope on the CD19 molecule, providing precise binding and minimizing off-target effects. This is particularly beneficial for applications requiring high specificity such as flow cytometry.
-
Does this scFv Fragment have any known half-life characteristics in biological systems?
A: While exact half-life data might vary, scFv fragments usually have a shorter half-life than full antibodies. However, they can be engineered for extended stability, and pilot pharmacokinetic studies are recommended.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 25C1"
- CAT
- Product Name
See other products for "CD19"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H17 | Anti-Human CD19 Recombinant Antibody (TAB-H17) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-1625CL | Anti-Human CD19 Recombinant Antibody (396) | Depletion, ELISA | Humanized antibody |
| TAB-1612CL-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1612CL-S(P)) | Depletion, FuncS | Humanized scFv |
| TAB-1625CL-S(P) | Anti-Human CD19 Recombinant Antibody scFv Fragment (396) | Depletion, ELISA | Humanized antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L047E | anti-CD19 immunotoxin HD37 (scFv)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L047D | anti-CD19 immunotoxin HD37 (scFv)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L047R | anti-CD19 immunotoxin HD37 (scFv)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L047S | anti-CD19 immunotoxin HD37 (scFv)-Sap | Cytotoxicity assay, Functional assay | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-108CL | Anti-Human CD19 Recombinant Antibody (TAB-108CL) | FC, ADCC, FuncS | Antibody |
| TAB-1616CL-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1616CL-S(P)) | ELISA, WB | Human scFv |
| TAB-1617CL-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1617CL-S(P)) | ELISA, WB | Human scFv |
| TAB-1618CL-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1618CL-S(P)) | ELISA, WB | Human scFv |
| TAB-1619CL-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1619CL-S(P)) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1611CL-S(P) | Mouse Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1611CL-S(P)) | Depletion, FuncS | Mouse scFv |
| TAB-1620CL-S(P) | Anti-Human CD19 Recombinant Antibody scFv Fragment (HD37) | FuncS | |
| TAB-1622CL-S(P) | Mouse Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1622CL-S(P)) | ELISA, FC, Apop, ADCC, FuncS | Mouse scFv |
| TAB-1623CL-S(P) | Mouse Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1623CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-1624CL-S(P) | Anti-Human CD19 Recombinant Antibody scFv Fragment (381) | FC, Inhib, ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1613CL-S(P) | Mouse Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1613CL-S(P)) | Depletion, FuncS | Mouse scFv |
| TAB-1614CL-S(P) | Mouse Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1614CL-S(P)) | FuncS | Mouse scFv |
| TAB-1621CL-S(P) | Anti-Human CD19 Recombinant Antibody scFv Fragment (cHD37) | FuncS | Chimeric antibody (mouse/human) |
| TAB-1613CL-F(E) | Human Anti-CD19 Recombinant Antibody; Fab Fragment (TAB-1613CL-F(E)) | Depletion, FuncS | Chimeric (mouse/human) Fab |
| TAB-1614CL-F(E) | Human Anti-CD19 Recombinant Antibody; Fab Fragment (TAB-1614CL-F(E)) | FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-061LC | Recombinant Anti-Human CD19 Antibody (Fc glycosylation/Low fucosylated) | ELISA, FC | Chimeric antibody (mouse/human) |
| Gly-105LC | Recombinant Anti-Human CD19 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
| Gly-141LC | Recombinant Anti-Human CD19 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-061LC-1 | Recombinant Anti-Human CD19 Antibody (Fc glycosylation/Low fucosylated) | ELISA, FC | Chimeric antibody (mouse/human) |
| Gly-141LC-1 | Recombinant Anti-Human CD19 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0278MZ | Mouse Anti-CD19 Recombinant Antibody (clone ME-CD20) | IHC | Mouse IgG1 |
| HPAB-0264-CN | Human Anti-CD19 Recombinant Antibody (HPAB-0264-CN) | ELISA, FC | Humanized IgG1, κ |
| HPAB-0265-CN | Human Anti-CD19 Recombinant Antibody (HPAB-0265-CN) | ELISA, FC | Humanized IgG1, κ |
| HPAB-0266-CN | Human Anti-CD19 Recombinant Antibody (HPAB-0266-CN) | ELISA, FC | Humanized IgG |
| HPAB-0267-CN | Human Anti-CD19 Recombinant Antibody (HPAB-0267-CN) | ELISA, FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0100MZ | Chicken Anti-CD19 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-286CQ | Mouse Anti-CD19 Recombinant Antibody (clone SJ25-C1) | Neut, FC, IHC, IHC-Fr, IP | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-288CQ | Rat Anti-CD19 Recombinant Antibody (clone 1D3) | Depletion, Block, FC | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0538 | Hi-Affi™ Rabbit Anti-CD19 Recombinant Antibody (clone DS538AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0264-CN-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (HPAB-0264-CN-S(P)) | ELISA, FC | Humanized scFv |
| HPAB-0265-CN-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (HPAB-0265-CN-S(P)) | ELISA, FC | Humanized scFv |
| HPAB-0266-CN-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (HPAB-0266-CN-S(P)) | ELISA, FC | Humanized scFv |
| HPAB-0267-CN-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (HPAB-0267-CN-S(P)) | ELISA, FC | Humanized scFv |
| HPAB-0268-CN-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (HPAB-0268-CN-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0459LY-F(E) | Human Anti-CD19 Recombinant Antibody (clone 1); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0460LY-F(E) | Human Anti-CD19 Recombinant Antibody (clone 2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0461LY-F(E) | Human Anti-CD19 Recombinant Antibody (clone 3); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0463LY-F(E) | Mouse Anti-CD19 Recombinant Antibody (clone CBL002LY); Fab Fragment | FC, in vitro cytotoxic, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY59 | Human Anti-CD19 (clone 2A3) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY1127 | Anti-Rat CD19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1128 | Anti-Mouse CD19 Immunohistochemistry Kit | IHC |
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