Human Anti-CD274 Recombinant Antibody (clone Lesabelimab) (CAT#: VS-0624-FY44)
CD274 is an immune checkpoint ligand, represses antitumour immunity through the interaction with PDCD1 receptor. Lesabelimab (LDP) is an immunoglobulin G1-kappa anti-CD274 monoclonal antibody.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Figure 4 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
Specifications
- Host Species
- Human
- Type
- Human IgG1 kappa
- Specificity
- Human CD274
- Species Reactivity
- Human
- Clone
- Lesabelimab
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 100 mM Pro-Ac, 20 mM Arg, pH 5.0
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1
- Gene ID
- 29126
- UniProt ID
- Q9NZQ7
- Long Name
- Programmed death-ligand 1
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Cellular Localization
- Cell membrane, Endosome, Membrane, Nucleus, Secreted
- Post Translation Modifications
- Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation (PubMed:28813410).
Ubiquitinated by MARCHF8; leading to degradation (PubMed:34183449).
Glycosylation at Asn35, Asn192, Asn200, and Asn219 (NX_Q9NZQ7 [NX_Q9NZQ7-1])
Modification sites at PhosphoSitePlus (Q9NZQ7)
Glycosylation from GlyGen (Q9NZQ7) 5 sites
- Protein Refseq
- XP_047279218.1; XP_054218785.1; NP_001254635.1; NP_001300958.1; NP_054862.1
- Function
- Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410).
As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| HPAB-1440LY-F(E) | Human Anti-CD274 Recombinant Antibody Fab Fragment (HPAB-1440LY-F(E)) | IHC | Chimeric (Rabbit/Human) Fab |
| HPAB-0400LY-F(E) | Human Anti-PDCD1 Recombinant Antibody; Fab Fragment (HPAB-0400LY-F(E)) | ELISA, RIA, WB | Human Fab |
| HPAB-0401LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0401LY-F(E)) | ELISA, RIA, WB | Human Fab |
| HPAB-0402LY-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0402LY-F(E)) | ELISA, RIA, WB | Human Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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