Human Anti-CD274 Recombinant Antibody (clone H1B12+L5A10); scFv Fragment (CAT#: HPAB-M0297-YC-S(P))
This product is a scFv fragment of an antagonism PDL1 monoclonal antibody. Its full-length antibody has a high affinity binding to PD-L1 with the KD value of 1.5 nM as tested by SPR, a good sensitivity with an EC₅₀ value of 0.25 nM tested by ELISA, and a good inhibiting ability with an IC₅₀ value of 8 nM. It could be used in immunoassays and PD-L1 related immunotherapy research.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Figure 4 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
Specifications
- Host Species
- Human
- Type
- Human scFv
- Specificity
- Human CD274
- Species Reactivity
- Human
- Clone
- H1B12+L5A10
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1;
- Gene ID
- 29126
- UniProt ID
- Q9NZQ7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone H1B12+L5A10"
See other products for "Cd274"
Select a product category from the dropdown menu below to view related products.
Please select product type
ScFv Antibodies
Mouse Antibodies
Chimeric Antibodies
Human Antibodies
Humanized Antibodies
IgG Antibodies
Neutralizing Antibodies
Blocking Antibodies
Rabbit Monoclonal Antibodies
Single Domain Antibodies
Fab Antibodies
ADCC Enhanced Antibodies
Hexamer Antibodies
NHP Antibodies
FC Reagents & Kits
ScFv-Fc Chimeras
Extracellular Vesicle Antibodies
IHC Kits & Antibodies
Biparatopic Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-0561LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0561LY-S(P)) | ELISA, Block | Chimeric scfv |
| HPAB-0562LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0562LY-S(P)) | ELISA, Block | Chimeric scfv |
| HPAB-0563LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0563LY-S(P)) | ELISA, Block | Chimeric scfv |
| HPAB-0564LY-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0564LY-S(P)) | ELISA, Block | Chimeric scfv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-M0297-YC-S(P). Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, Neut, FuncS
-3.png)
Application: ELISA, FC, Block, SPR
-2.png)
Application: IB, ELISA, FC, FuncS
Application: WB, ELISA, IF
Application: ELISA, IF, Block, FuncS
Application: ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
