Mouse Anti-CD44 Recombinant Antibody (clone 6G2D2) (CAT#: ZG-0237U)

Mouse anti-CD44 antibody (clone 6G2D2) displayed high specificity against the CD44 protein of Human.

Specific Inquiry
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  • Tested Data
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WB

Figure 1 Mouse Anti-CD44 Antibody (ZG-0237U) in WB

Figure 1 Mouse Anti-CD44 Antibody (ZG-0237U) in WB

Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate All lanes: CD44 antibody at 1: 1500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa
Observed band size: 95 kDa

IHC

Figure 2 Mouse Anti-CD44 Antibody (ZG-0237U) in IHC

Figure 2 Mouse Anti-CD44 Antibody (ZG-0237U) in IHC

IHC image of ZG-0237U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IF

Figure 3 Mouse Anti-CD44 Antibody (ZG-0237U) in IF

Figure 3 Mouse Anti-CD44 Antibody (ZG-0237U) in IF

Immunofluorescence staining of Hela cells with ZG-0237U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

FC

Figure 4 Mouse Anti-CD44 Antibody (ZG-0237U) in FC

Figure 4 Mouse Anti-CD44 Antibody (ZG-0237U) in FC

Overlay histogram showing Hela cells stained with ZG-0237U (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.


Specifications

  • Immunogen
  • Recombinant Human CD44 antigen protein (21-220AA)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human CD44
  • Species Reactivity
  • Human
  • Clone
  • 6G2D2
  • Applications
  • ELISA, WB, IHC, IF, FC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Superficial Keratitis and Intraductal Papilloma.

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
    WB:1:1000-1:40000, IHC:1:50-1:200, IF:1:50-1:200

Target

  • Alternative Names
  • IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; CD44 antigen; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Indian blood group antigen; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; epican; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1; soluble CD44

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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