Mouse Anti-CD44 Recombinant Antibody (clone A3D8) (CAT#: HPAB-0141CQ)

This product is a recombinant mouse antibody that can bind to human CD44 with high affinity.

Specific Inquiry Online Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Inhib

Figure 1 H90 and A3D8 anti-CD44 mAbs inhibit proliferation of HL60, NB4, THP-1, and KG1a cell lines.

Figure 1 H90 and A3D8 anti-CD44 mAbs inhibit proliferation of HL60, NB4, THP-1, and KG1a cell lines.

A total of 10⁵ cells/mL were seeded into 96-well plates and incubated in triplicate in the presence of specific anti-CD44 mAbs (2.5 μg/mL A3D8 or 20 μg/mL H90). Controls were cells incubated with isotypic control (20 μg/mL IgG1). At the indicated times, the viable cell number was determined using trypan blue dye exclusion

Charrad, R. S., Gadhoum, Z., Qi, J., Glachant, A., Allouche, M., Jasmin, C.,... & Smadja-Joffe, F. (2002). Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines. Blood, 99(1), 290-299.

IF

Figure 2 Apoptotic cells, 3 days following addition of 2.5 μg/mL A3D8.

Figure 2 Apoptotic cells, 3 days following addition of 2.5 μg/mL A3D8.

Fluorescent staining with acridine orange and ethidium bromide showed nuclei condensation and fragmentation that are apoptosis-specific. Cells at an early stage of apoptosis (without loss of membrane integrity) displayed a bright green–stained nucleus. Apoptic cells that have lost membrane integrity displayed bright orange–stained nuclei as a result of taking up ethidium bromide.

Charrad, R. S., Gadhoum, Z., Qi, J., Glachant, A., Allouche, M., Jasmin, C.,... & Smadja-Joffe, F. (2002). Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines. Blood, 99(1), 290-300.

FC

Figure 3 Apoptotic cells, 3 days following addition of 2.5 μg/mL A3D8.

Figure 3 Apoptotic cells, 3 days following addition of 2.5 μg/mL A3D8.

DNA strand breaks, which are apoptosis-specific, were labeled using TUNEL reaction. Their amount was evaluated by flow cytometry analysis. The proportion of labeled (apoptotic) cells was measured by flow cytometry analysis relative to negative controls labeled with dUTP without terminal deoxynucleotidyl transferase.

Charrad, R. S., Gadhoum, Z., Qi, J., Glachant, A., Allouche, M., Jasmin, C.,... & Smadja-Joffe, F. (2002). Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines. Blood, 99(1), 290-301.

IHC

Figure 4 A3D8 induces monocytic differentiation of THP-1 and HL60 cells.

Figure 4 A3D8 induces monocytic differentiation of THP-1 and HL60 cells.

The data have been determined after addition of 2.5 μg/mL A3D8 at day 5 in the case of THP-1 cells.

Charrad, R. S., Gadhoum, Z., Qi, J., Glachant, A., Allouche, M., Jasmin, C.,... & Smadja-Joffe, F. (2002). Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines. Blood, 99(1), 290-302.

WB

Figure 5 A3D8 induces monocytic differentiation of THP-1 and HL60 cells.

Figure 5 A3D8 induces monocytic differentiation of THP-1 and HL60 cells.

Induction of synthesis of IL-1β, G-CSF, and IL-3 transcripts in HL60 cells. Total RNA from control and treated HL60 cells was prepared as described in Materials and methods. Using primers for glyceraldehyde phosphodehydrogenase, the amounts of cDNA were equilibrated to this internal standard. RT-PCR amplification products were separated by 1% agarose gel electrophoresis with 0.5 μg/mL ethidium bromide and were pictured under UV-light irradiation.

Charrad, R. S., Gadhoum, Z., Qi, J., Glachant, A., Allouche, M., Jasmin, C.,... & Smadja-Joffe, F. (2002). Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines. Blood, 99(1), 290-303.


Specifications

  • Immunogen
  • Circulating malignant human Sezary T cells
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human CD44
  • Species Reactivity
  • Human
  • Clone
  • A3D8
  • Applications
  • FC, IHC, IF, WB, Inhib

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Inhibition, Immunofluorescence, Flow Cytometry, Immunohistochemistry and Western Blot. Please see the Published Data for further information.

Target

  • Alternative Names
  • CD44 Molecule (Indian Blood Group); Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Hyaluronate Receptor; Hermes Antigen; CD44 Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone A3D8"

See other products for "CD44"

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0101MZ Chicken Anti-CD44 Polyclonal IgY WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-378CQ Rat Anti-CD44 Recombinant Antibody (clone 1M7.8.1) FC, IF, Inhib, IP, WB Rat IgG2b
NEUT-381CQ Human Anti-CD44 Recombinant Antibody (clone Hermes-3) ELISA, FC, IF, IHC, IP, Neut, WB Human IgG1, κ
NEUT-382CQ Rat Anti-CD44 Recombinant Antibody (clone IM7) Block Rat IgG2b
NEUT-383CQ Rat Anti-CD44 Recombinant Antibody (clone KM201), FITC Block, FC, IP, WB, FuncS Rat IgG1, κ
NEUT-384CQ Rat Anti-CD44 Recombinant Antibody (clone KM81) IF, IHC-P, FC, Block Rat IgG2a

Neutralizing Antibody

CAT Product Name Application Type
NEUT-379CQ Mouse Anti-CD44 Recombinant Antibody (clone F10-44-2) WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC Mouse IgG2a
NEUT-380CQ Rat Anti-CD44 Recombinant Antibody (clone Hermes-1) WB, Neut, ELISA, FC, IF, IHC, IP Rat IgG2a

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0563 Hi-Affi™ Rabbit Anti-CD44 Recombinant Antibody (clone DS563AB) FC, IF, IHC-Fr, IHC-P, WB Rabbit IgG
MOR-4548 Hi-Affi™ Rabbit Anti-CD44 Recombinant Antibody (clone TH58DS) IF, ICC, FC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-727 Afuco™ Anti-CD44 ADCC Recombinant Antibody (Bivatuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

Submit a review or a question
There are currently no Customer reviews or questions for HPAB-0141CQ. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

  • 0
  • 0
Cart
    Go to compare

    Go to compare