Mouse Anti-CD44 Recombinant Antibody (clone HI44a) (CAT#: HPAB-0047-YJ)

Figure 1 Modulation of nitroblue tetrazolium (NBT)-reducing ability of different types of acute myeloid leukemia cells by HI44a, a monoclonal antibody against CD44.

Leukemia cells were incubated with HI44a at 10-50 μg/ml. The percentage of NBT+ cells was evaluated. Results are shown as the mean (±S.D.) of specific subtype cases.

Song, G., Liao, X., Zhou, L., Wu, L., Feng, Y., & Han, Z. C. (2004). HI44a, an anti-CD44 monoclonal antibody, induces differentiation and apoptosis of human acute myeloid leukemia cells. Leukemia research, 28(10), 1089-1096.

Figure 2 HI44a-induced differentiation of AML-M2 to AML-M5 leukemia cells.

Cells were incubated with 10-50 μg/ml HI44a. Treated cells and controls were stained with PE-conjugated anti-CD11b monoclonal antibody and FITC-conjugated anti-CD14 (monocyte-specific) or anti-CD15 (granulocyte-specific) monoclonal antibody. The %CD11b+, CD14+, or CD15+ cells was determined by flow cytometry. (a) Simultaneous increased expression of CD11b and CD14; (b) increased expression of CD15.

Song, G., Liao, X., Zhou, L., Wu, L., Feng, Y., & Han, Z. C. (2004). HI44a, an anti-CD44 monoclonal antibody, induces differentiation and apoptosis of human acute myeloid leukemia cells. Leukemia research, 28(10), 1089-1096.

Figure 3 HI44a induced change of cytological features in AML-M3 and AML-M5 cells.

Song, G., Liao, X., Zhou, L., Wu, L., Feng, Y., & Han, Z. C. (2004). HI44a, an anti-CD44 monoclonal antibody, induces differentiation and apoptosis of human acute myeloid leukemia cells. Leukemia research, 28(10), 1089-1096.

Figure 4 FITC-annexin-V evaluation of early apoptotic cells. Early apoptotic cells are FITC-positive and PI-negative.

Song, G., Liao, X., Zhou, L., Wu, L., Feng, Y., & Han, Z. C. (2004). HI44a, an anti-CD44 monoclonal antibody, induces differentiation and apoptosis of human acute myeloid leukemia cells. Leukemia research, 28(10), 1089-1096.