PTM™ Rabbit Anti-CDK2 (pTyr15) Recombinant Antibody (VS13-YC196) (CAT#: VS13-YC196)

• Immunogen
• Synthetic phosphopeptide of human Cdk2 (pTyr15).

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• The phosphorylated CDK2 (Tyr15) of human, hamster and rat.

• Species Reactivity
• Human, Hamster, Rat

• Applications
• WB, IP

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Affinity Chromatography purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.

• Preservative
• 0.01% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunoprecipitation (IP): 1/20

• Alternative Names
• Cyclin Dependent Kinase 2; Cell Division Protein Kinase 2; P33 Protein Kinase; EC 2.7.11.22; CDKN2; CDC2-Related Protein Kinase; Cyclin-Dependent Kinase 2; P33(CDK2); EC 2.7.11;

• Gene ID
• 1017

• UniProt ID
• P24941

• Long Name
• Cyclin Dependent Kinase 2

• Sequence Similarities
• Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

• Cellular Localization
• Cytoplasm; Cytoskeleton; Endosome; Nucleus

• Post Translation Modifications
• Phosphorylated

• Protein Refseq
• NP_001277159.1; NP_001789.2; NP_439892.2

• Function
• Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity).
  Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity).
  Phosphorylates CDK2AP2 (PubMed:12944431).
  Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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