AbPlus™ Anti-UBE2H Magnetic Beads (VS-0724-YC1165) (CAT#: VS-0724-YC1165)
The AbPlus Anti-UBE2H Magnetic Beads (VS-0724-YC1165) is an innovative affinity resin which is bound with anti-UBE2H specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2H protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/if_selected.jpg

Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/ihc_selected.jpg

Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_B_8_5.jpg

Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_7_4.jpg

Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_8_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_4_6.jpg

Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3302/9836_A_8_8.jpg

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186591-UBE2H
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186591-UBE2H
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE2H specific antibody
- Target
- UBE2H
- Immunogen
- Recombinant human UBE2H, aa 1-183.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 H; Ubiquitin-Conjugating Enzyme E2H (UBC8 Homolog, Yeast); (E3-Independent) E2 Ubiquitin-Conjugating Enzyme H; Ubiquitin-Conjugating Enzyme E2-20K; E2 Ubiquitin-Conjugating Enzyme H; Ubiquitin Carrier Protein H; Ubiquitin-Protein Ligase H; UBCH2; Ubiquitin-Conjugating Enzyme E2H (Homologous To Yeast UBC8); GID Complex Subunit 3, UBC8 Homolog (S. Cerevisiae); GID Complex Subunit 3, UBC8 Homolog;
- Gene ID
- 7328
- UniProt ID
- P62256
Downloads
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IgG Antibody Products
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1205MZ | Recombinant Mouse Anti-Human UBE2H Antibody | ICC/IF, WB | Mouse antibody |
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