GeneSpark™ Human ID1 Promoter Primers (VS-0725-FY33)
CAT#: VS-0725-FY33
The Human ID1 Promoter Primers offer a precise tool for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product provides a pre-mixed set of forward and reverse PCR primers specifically targeting the promoter region of the human ID1 (Inhibitor of DNA binding 1) gene. ID1 is a key regulator in cell proliferation, differentiation, and angiogenesis, often overexpressed in various cancers. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been rigorously tested with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are ideal for investigating transcriptional regulation and protein-DNA interactions at this crucial promoter.
Specifications
- Application
- ChIP
- Application Notes
- The core function of primers in ChIP experiments is to serve as specific probes for detecting and quantifying particular DNA fragments enriched after immunoprecipitation, thereby revealing protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Inhibitor Of DNA Binding 1, HLH Protein
- Introduction
- The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- Inhibitor Of DNA Binding 1, HLH Protein
- Gene ID
- 3397
- UniProt ID
- P41134
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