GeneSpark™ Human IFN-γ Promoter Primers (VS-0725-FY37)
CAT#: VS-0725-FY37
The Human IFN-γ Promoter Primers are designed for amplifying DNA isolated through chromatin immunoprecipitation (ChIP). This product includes optimized forward and reverse PCR primers specific to the promoter region of the human IFN-γ (Interferon-gamma) gene. IFN-γ is a pivotal cytokine in innate and adaptive immunity, playing a critical role in antiviral and antitumor responses. Its expression is tightly regulated at the promoter level. These primers are optimized for SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are ideal for investigating epigenetic regulation during immune responses.
Specifications
- Application
- ChIP
- Application Notes
- The core function of primers in ChIP experiments is to serve as specific probes for detecting and quantifying particular DNA fragments enriched after immunoprecipitation, thereby revealing protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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