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    Recombinant Anti-SEMA4B Vesicular Antibody, EV Displayed (VS-0425-YC123) (CAT#: VS-0425-YC123)

    The Recombinant Anti-SEMA4B Vesicular Antibody, EV Displayed (VS-0425-YC123) is an antibody-displaying extracellular vesicle (Ab-EV). The product combines the benefits of both extracellular vesicle (EV) and antibody (Ab) which can guide the decorated EVs to SEMA4B-expressed cells or tissues. The SEMA4B is Semaphorin 4B, a protein-coding gene involved in apoptotic pathways in synovial fibroblasts and PAK pathways. It is important in receptor activity and related pathways, with SEMA4A as an important paralog.

    Specific Inquiry
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line RT4
    Figure 1 IF staining of human cell line RT4
    (Immunofluorescent staining of human cell line RT4 shows localization to cell junctions.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/1596_D1_3_selected.jpg
    Figure 2 IHC staining of human small intestine
    Figure 2 IHC staining of human small intestine
    (Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/ihc_selected.jpg
    Figure 3 IF staining of human cell line A549
    Figure 3 IF staining of human cell line A549
    (Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/1584_C2_1_blue_red_green.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/30766_B_8_5.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex

    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/30766_B_8_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/30766_A_9_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Hepatocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/30766_A_7_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/30766_A_9_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13372/30766_A_5_6.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enhanced (CAPAN-2, RPTEC TERT1, RT4)
    Figure 10 RNA cell line category: Cell line enhanced (CAPAN-2, RPTEC TERT1, RT4)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185033-SEMA4B

    Recombinant Antibody

    • Application
    • ELISA, FC, Cell-uptake
    • Product Type
    • Ab-Fc-EVs
    • Antibody Quantification (Ab/EV)
    • ~100 Ab/EV
    • Target
    • SEMA4B
    • Host Animal
    • Human
    • Antibody Isotype
    • IgG
    • Species Reactivity
    • Human
    • Expression Cell
    • Mammalian cell

    Engineered EVs

    • EV-sorting domain
    • CD63
    • Fc-binding domain
    • z domain
    • EV Size
    • 30~150 nm
    • Producing Cell
    • HEK293F
    • Isolation Method
    • Gradient centrifugation
    • Purification
    • qEV size exclusion chromatography
    • Binding Affinity
    • Kd = 0.85 µg/mL
    • Concentration
    • 1 x 10¹⁰
    • Size
    • 1 mL
    • Buffer
    • PBS
    • Storage
    • Store at -80°C for 12 months

    Target

    • Full Name
    • Semaphorin 4B
    • Biological Process
    • Differentiation, Neurogenesis
    • Molecular Function
    • Developmental protein
    • Cellular Localization
    • Cell Junctions, Nucleoplasm, Vesicles
    • Introduction
    • SEMA4B (Semaphorin 4B) is a Protein Coding gene. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and PAK Pathway. Gene Ontology (GO) annotations related to this gene include receptor activity. An important paralog of this gene is SEMA4A.
    • Alternative Names
    • KIAA1745, MGC131831, SEMAC, SemC

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    Single Domain Antibodies IgG Antibodies
    CAT Product Name Application Type
    NAB-2274-VHH Recombinant Anti-Human SEMA4B VHH Single Domain Antibody FUNC, FA, IP, ChiP Llama VHH

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