Swift™ ChIP Kit (Plants) (VS-0725-FY3)
CAT#: VS-0725-FY3
Plants ChIP Kit is an advanced tool designed to quickly identify direct, genome-wide associations between regulatory proteins and their target genes in plant cells. Essential for understanding cellular functions like signal transduction and gene expression, this kit allows for efficient chromatin immunoprecipitation within six hours. It's compatible with various downstream applications, including qualitative and quantitative PCR, MS-PCR, DNA sequencing, Southern blot, and DNA microarrays. The kit supports 24 or 48 ChIP reactions, with a standard protocol performing 8 reactions per sample, offering flexibility for researchers to adjust sample and reagent amounts for more extensive studies.
Specifications
- Application
- ChIP
- Workflow
- 1. Cell/Tissue Preparation & Cross-linking: Cells or tissues are collected, and DNA-protein interactions are fixed using a cross-linking agent like formaldehyde, followed by quenching with glycine.
2. Cell Lysis & Chromatin Shearing: Cells undergo lysis, and chromatin is mechanically sheared (e.g., via sonication) into smaller fragments, typically 100-1000 bp. Sheared chromatin is then separated from debris by centrifugation.
3. Magnetic Immunoprecipitation: Protein A-coated magnetic beads are washed and then incubated with sheared chromatin and a specific antibody (or control IgG) to capture antibody-protein-DNA complexes.
4. Washing: Non-specifically bound components are removed by extensively washing the immunocomplex-bound beads with various buffers.
5. Elution, Decross-linking & DNA Purification: Immunoprecipitated protein-DNA complexes are eluted. Cross-links are reversed by high-temperature incubation with proteinase K, releasing the DNA, which is then purified.
6. Downstream Analysis: The purified DNA is analyzed to identify and quantify specific bound sequences using methods such as quantitative PCR (qPCR) or Next-Generation Sequencing (ChIP-seq).
- Size
- 24 Tests
- Components
- 100X Protease Inhibitor Cocktail: 1 x 25 µL
5X Lysis Buffer I: 1 x 12 mL
8-Well Assay Strips (with Frame): 3 x 1 Unit
8-Well Strip Caps: 3 x 1 Unit
Binding Buffer: 1 x 5 mL
ChIP Dilution Buffer: 1 x 2 mL
DNA Release Buffer: 1 x 2 mL
Elution Buffer: 1 x 0.6 mL
F-Collection Tube: 30 x 1 Unit
F-Spin Column: 30 x 1 Unit
Lysis Buffer II: 1 x 3 mL
Lysis Buffer III: 1 x 2 mL
Lysis Buffer IV: 1 x 1.5 mL
Proteinase K (10 mg/mL): 1 x 25 µL
Reverse Buffer: 1 x 2 mL
Wash Buffer: 1 x 28 mL
Reagent composition can vary by lot. Contact us for further parameter details.
- Positive Control
- Anti-H3K9me2: 1 x 5 µL
Antibody Buffer: 1 x 15 mL
- Negative Control
- Normal Mouse IgG: 1 x 10 µL
- Biological Samples
- Plants
- Shipping Conditions
- Blue Ice
- Storage
- All components within this kit remain stable for at least 6 months beyond the reference date on their labels, provided they are stored at the recommended temperature and remain unused.
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