sfGFP

Waldo and other research groups has reported the engineering of a super folder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding. sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments. Furthermore, sfGFP fusions are more soluble than conventional GFP fusions.