Recombinant Mouse Anti-Aspartic protease Bla g 2 Antibody (7C11) (CAT#: PABL-016)
Recombinant Mouse Antibody (7C11) is capable of binding to Aspartic protease Bla g 2, expressed in Chinese Hamster Ovary cells (CHO).
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ELISA
Figure 1 Dose-response curves of Bla g 2 and epitope mutants by ELISA.
Binding of the anti-Bla g 2 polyclonal antibody to mAb 7C11 epitope mutants.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7).
Inhib
Figure 2 Inhibition of biotinylated mAb binding to rBla g 2 by allergen mutants using ELISA.
Inhibition of binding of biotinylated-mAb 7C11 to rBla g 2-N93Q by mAb 7C11 epitope mutants.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7).
FuncS
Figure 3 Binding of IgE antibodies from cockroach allergic patients to rBla g 2-N93Q and epitope mutants.
IgE binding to mAb7C11 epitope mutants.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7).
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native B. germanica Aspartic protease Bla g 2
- Species Reactivity
- B. germanica
- Clone
- 7C11
- Applications
- ELISA, Inhib, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Inhibition and Function Assay.
Target
- Alternative Names
- Aspartic protease Bla g 2; Allergen Bla g II; Allergen: Bla g 2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-016 | Recombinant Mouse Anti-Aspartic protease Bla g 2 Antibody scFv Fragment (7C11) | WB, ELISA, IF, FuncS | scFv |
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