Recombinant Rat Anti-FPV Antibody (mAbE)
CAT#: PABW-139
Recombinant Rat Antibody (mAbE) is capable of binding to FPV, expressed in Chinese Hamster Ovary cells (CHO). MAbE neutralized virus at a low ratio of only 25 Fabs per capsid and inhibited binding of TfR to the virus.
Published Data
IB
Figure 1 Purified felinized mAb E is expressed and assembles correctly.
SDS-PAGE (A,B) and Immunoblot (C,D) analysis of felinized mAb E under non-reducing (A) and reducing conditions (B–D). One µg of protein was loaded per lane. Proteins in gels (A,B) were visualized by Coomassie blue staining. PVDF membranes (C,D) were incubated with HRP-conjugated goat anti-cat Ig or goat anti-rat Ig antibodies, respectively.
Lu, Z., Tallmadge, R. L., Callaway, H. M., Felippe, M. J. B., & Parker, J. S. (2017). Sequence analysis of feline immunoglobulin mRNAs and the development of a felinized monoclonal antibody specific to feline panleukopenia virus. Scientific reports, 7(1), 12713.
Inhib
Figure 2 Felinized mAb E inhibits hemagglutination by (A) CPV and (B) FPV.
Eight HA units of purified CPV or FPV empty capsids were added to two-fold serial diluted antibodies starting at 80 µg/ml. After incubation at room temperature for 1 hr, 50 µl of 0.5% feline red blood cells were added to each well and the plates were incubated at 4 °C overnight. Experiments were done three times independently. A representative plate is shown.
Lu, Z., Tallmadge, R. L., Callaway, H. M., Felippe, M. J. B., & Parker, J. S. (2017). Sequence analysis of feline immunoglobulin mRNAs and the development of a felinized monoclonal antibody specific to feline panleukopenia virus. Scientific reports, 7(1), 12713.
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Specifications
- Immunogen
- FPV
- Host Species
- Rat
- Derivation
- Rat
- Type
- IgG
- Specificity
- Tested positive against native FPV
- Species Reactivity
- FPV
- Clone
- mAbE
- Applications
- Neut, IB, Inhib
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The FPV antibody has been reported in applications of Neutralization, Immunoblotting, Inhibition.
For Inhibition: To test the function of the engineered felinized mAb E, HAIs were used to determine the capacity of mAbs to inhibit CPV or feline panleukopenia virus (FPV) hemagglutination of feline red blood cells. Antibodies were serially diluted (12.5 μl of 80 μg ml−1 mAb E; felinized mAb E; or feline parental IgG-IgK) 1:1 in Bis-Tris buffered saline (BTBS; 0.1 mol Bis-Tris Base, 0.15 mol NaCl, 2.5 mmol MgCl₂, 0.75 mmol CaCl₂ and 0.1% BSA, pH = 5.87). Eight HA units of purified CPV or FPV empty capsids in 12.5 μl PBS were added to each well (25 μl total volume) in a 96-well V-bottom plate, and incubated for 1 h at room temperature. Feline red blood cells (0.5% in BTBS; 50 µl) were added to each well, and the plates were further incubated at 4 °C for 2 hours or overnight before being read.
Target
- Alternative Names
- FPV; Feline parvovirus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-138 | Recombinant Rat Anti-FPV Antibody (mAbF) | Neut | IgG |
| PABW-144 | Recombinant Rat Anti-FPV Antibody (mAbB) | Neut | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-138 | Recombinant Rat Anti-FPV Antibody Fab Fragment (mAbF) | Neut | Fab |
| PFBW-139 | Recombinant Rat Anti-FPV Antibody Fab Fragment (mAbE) | Neut | Fab |
| PFBW-144 | Recombinant Rat Anti-FPV Antibody Fab Fragment (mAbB) | Neut | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-138 | Recombinant Rat Anti-FPV Antibody scFv Fragment (mAbF) | Neut | scFv |
| PSBW-139 | Recombinant Rat Anti-FPV Antibody scFv Fragment (mAbE) | Neut | scFv |
| PSBW-144 | Recombinant Rat Anti-FPV Antibody scFv Fragment (mAbB) | Neut | scFv |
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