Recombinant Anti-human G3BP Intrabody [Tat] (CAT#: IAB-B016(T))
Recombinant anti-G3BP intrabody in fusion with HIV Tat works within cells to bind intracellur antigen human G3BP, expressed in 293T cells. This inhibition is attributed to the blocking of the endogenous G3BP protein.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/if_selected.jpg

(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/27_D12_1_blue_red_green.jpg

(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/26_D12_1_blue_red_green.jpg

(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/ihc_selected.jpg

(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37068/ihc_selected.jpg

(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_B_7_5.jpg

(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_9_3.jpg

(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_8_5.jpg

(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_4_6.jpg

(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4052/151830_A_9_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145907-G3BP1
Specifications
- Immunogen
- human GTPase activating protein (SH3 domain) binding protein 1
- Host Species
- llama
- Type
- vhh-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Products can be used for: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- Short Term Storage: 4°C
Long Term Storage: -20°C
Target
- Alternative Names
- G3BP1; GTPase activating protein (SH3 domain) binding protein 1; G3BP; HDH-VIII; ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuc
- Gene ID
- 10146
- UniProt ID
- Q13283
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "G3BP"
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CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B016(A) | Recombinant Anti-human G3BP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | vhh-(D-Arg)9 |
IAB-B016(G) | Recombinant Anti-human G3BP Intrabody [+36 GFP] | WB, CO-IP, FuncS | vhh-(+36GFP) |
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For Research Use Only. Not For Clinical Use.
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