Recombinant Mouse anti-Human POLR3E Monoclonal antibody (3802D4b) (CAT#: MOB-1619CT)
Recombinant Mouse monoclonal antibody to Human POLR3E, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/if_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/ihc_selected.jpg
(Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_B_7_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_8_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_8_4.jpg
(Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_4_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_4_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_7_8.jpg
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41477/92534_A_7_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000058600-POLR3E/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000058600-POLR3E/subcellular
Specifications
- Immunogen
- Recombinant fragment with a tag, corresponding to internal sequence amino acids 92-208 of Human POLR3E.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 3802D4b
- Applications
- Used for immunoassay techniques such as: Dot blot; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.1 mg/ml
- Buffer
- Preservative: 0.05% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- POLR3E; polymerase (RNA) III (DNA directed) polypeptide E (80kD); SIN; RPC5; DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; RNA polymerase III 80 kDa subunit RPC5; DNA-directed RNA polymerase III 80 kDa polypeptide;
- Gene ID
- 55718
- UniProt ID
- Q9NVU0
- Protein Refseq
- NP_060589
- Function
- DNA-directed RNA polymerase activity;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC130 | AbPlus™ Anti-POLR3E Magnetic Beads (VS-0724-YC130) | IP, Protein Purification |
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