Anti-Human RSV-G Recombinant Antibody (131-2G) (CAT#: TAB-372CT)
Recombinant monoclonal antibody specifically binds to a conserved epitope of the G protein of RSV. It can be potentially used in treating RSV infections.
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Inhib
Figure 1 mAb 232-1F was examined for its ability to block RSV G protein induced leukocyte migration in vitro compared to mAb 232-1F and mAb 131-2G.
An anti-RSV F antibody (nIg) was used a negative control. Data is expressed as percent inhibition of leukocyte chemotaxis.
Caidi, H., Harcourt, J. L., Tripp, R. A., Anderson, L. J., & Haynes, L. M. (2012). Combination therapy using monoclonal antibodies against respiratory syncytial virus (RSV) G glycoprotein protects from RSV disease in BALB/c mice. PloS one, 7(12), e51485.
Neut
Figure 2 Effect of MAb 131-2G prophylaxis on neutralizing antibody responseto r19F infection in BALB/c mice.
BALB/c mice were treated with intact (MAb Intact+r19F) or F(ab')₂[MAb F(ab')2+r19F] 131-2G 2 days before infectionwith 1×10⁶ TCID₅₀ of r19F (r19F) or mock-infected tissue culture material (mock). Neutralizing antibody titers were determined at 30±3, 45±3, 75±3, and 95±3 days after infection by a microneutralization assay with r19F.
Boyoglu-Barnum, S., Chirkova, T., Todd, S. O., Barnum, T. R., Gaston, K. A., Jorquera, P., ... & Anderson, L. J. (2014). Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice. Journal of virology, 88(18), 10569-10583.
FC
Figure 3 Effect of anti-RSV G protein MAb 131-2G treatment on the frequency of Tfh cells.
Representative dot plots from flow cytometric analysis of Bcl-6.
Boyoglu-Barnum, S., Chirkova, T., Todd, S. O., Barnum, T. R., Gaston, K. A., Jorquera, P., ... & Anderson, L. J. (2014). Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice. Journal of virology, 88(18), 10569-10583.
FuncS
Figure 4 Effect of anti-RSV G protein MAb 131-2G treatment on the frequency of peptide-induced T-bet and IFN-γor Gata-3 and IL-4 secretion in CD8 cells.
Spleen cells were harvested at day 75 p.i. and stimulated with the peptidesor ovalbumin (Ova) control peptide. Error bar represents the SEM from n=5 mice per group.
Boyoglu-Barnum, S., Chirkova, T., Todd, S. O., Barnum, T. R., Gaston, K. A., Jorquera, P., ... & Anderson, L. J. (2014). Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice. Journal of virology, 88(18), 10569-10583.
Specifications
- Host Species
- Humanized
- Derivation
- 131-2G hybridoma cell line
- Type
- Humanized antibody
- Specificity
- RSV
- Species Reactivity
- RSV
- Clone
- 131-2G
- Applications
- Neut, Inhib, FC, FuncS
- Related Disease
- RSV infection
Applications
- Application Notes
- This antibody has been reported for use in Neutralization, Inhibition, Flow Cytometry and Function Assay.
Target
- Alternative Names
- RSV-G; Respiratory Syncytial Virus G protein; anti-RSV-G
Related Resources
Cui, Guanglin, et al. "Preliminary functional and phylogeographic analyses of the 72 nucleotide duplication region in the emerging human respiratory syncytial virus ON1 strain attachment glycoprotein gene." Biomedicine & Pharmacotherapy 123 (2020): 109800. https://doi.org/10.1016/j.biopha.2019.109800
This research examined the 72-nucleotide duplication in the G gene of the emerging ON1 strain of human respiratory syncytial virus (hRSV). Using pseudotype lentiviral systems, the study directly investigated how this duplication affects viral infection processes. The researchers found that the lentiviral pseudoparticles effectively mimicked key G-protein functions during hRSV cell entry, and notably, deletion of the G-protein duplication significantly decreased infection efficiency. Additionally, phylogeographic analyses of 199 representative ON1 sequences revealed that ON1 isolates likely originated in Europe around 1998, with a nucleotide substitution rate of 2.14 × 10⁻³ substitutions per site per year in the second mucin-like highly variable region of the G-protein gene.
Creative Biolabs supplied anti-G mono-antibody 131-2G (TAB-372CT) and anti-F mono-antibody (TAB-358CT) for this study, which were essential for the immunoblotting and antibody blockade experiments. These antibodies enabled the researchers to validate the incorporation of G and F proteins into pseudoparticles and confirm receptor usage, demonstrating that the lentiviral system accurately replicated authentic hRSV attachment mechanisms. This contribution was instrumental in establishing the enhanced attachment function of the duplicated region in the ON1 G gene, helping explain this strain's rapid global spread and replacement of previously dominant genotypes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Humanized Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-372CT-S(P) | Anti-Human RSV-G Recombinant Antibody scFv Fragment (131-2G) | ELISA, WB, Neut | Humanized antibody |
| TAB-372CT-F(E) | Anti-Human RSV-G Recombinant Antibody Fab Fragment (131-2G) | ELISA, WB, Neut | Humanized antibody |
Customer Reviews and Q&As
Customer Review
Q&As
Laura Scott
Excellent for RSV G Studies
Excellent for RSV G Studies
The Anti-RSV G Therapeutic Antibody has been excellent for our RSV G studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
18.Oct,23
David Young
Reliable in Multiple Assays
Reliable in Multiple Assays
We incorporated this antibody into our Western blot and immunoassays, and it has been a great asset. The specificity for RSV G is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
05.Dec,22
Megan Martinez
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and high-quality in our various assays. The reproducible results and strong affinity for RSV G have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
09.Jul,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-372CT, RRID: AB_3111922)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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