What is the optimal dilution ratio for using clone M1709C9 in an ELISA assay?

For the Mouse Anti-V. cholerae O1 Ogawa & Inaba Recombinant Antibody (clone M1709C9), an ELISA assay's ideal dilution ratio is usually 1:500 to 1:2,000. To find the optimal concentration, a titration experiment tailored to the particular configuration is recommended.

What is the purity level of clone M1709C9, and how is it assessed?

The purity level of clone M1709C9 is typically greater than 95%, as assessed by SDS-PAGE and HPLC analysis. High purity ensures robust performance and reliability in various immunoassays.

How does clone M1709C9 compare to polyclonal antibodies in terms of specificity and reproducibility?

Clone M1709C9 offers higher specificity and reproducibility compared to polyclonal antibodies. As a monoclonal antibody, it targets a single epitope, reducing cross-reactivity and providing consistent results across different batches.

Mouse Anti-V. cholerae O1 Ogawa & Inaba Recombinant Antibody (clone M1709C9)

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Creative Biolabs provides state-of-the-art biosimilar development and biosimilar biosafety testing services for biologics. We tailor solutions to best suit your technical and budget requirements.

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Product Gene Keyword

Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Customer Reviews and Q&As

Customer Review Q&As

Fantastic for ELISA

The Mouse Anti-V. cholerae O1 Ogawa & Inaba Recombinant Antibody (clone M1709C9) worked excellently in our ELISA setup. The specificity and sensitivity were outstanding. I've used polyclonals before, but this monoclonal provides much cleaner results.

Impressive storage stability

The storage stability of clone M1709C9 is impressive. We had some antibody aliquots stored at -20°C for over six months, and they still performed reliably in all our assays.

Higher sensitivity

Our lab has conjugated clone M1709C9 to HRP for enhanced ELISA detection, and the results have been phenomenal. The sensitivity we achieved was much higher than with any other antibody we have used.

Q 1: What is the optimal dilution ratio for using clone M1709C9 in an ELISA assay?
A: For the Mouse Anti-V. cholerae O1 Ogawa & Inaba Recombinant Antibody (clone M1709C9), an ELISA assay's ideal dilution ratio is usually 1:500 to 1:2,000. To find the optimal concentration, a titration experiment tailored to the particular configuration is recommended.
Q 2: What is the purity level of clone M1709C9, and how is it assessed?
A: The purity level of clone M1709C9 is typically greater than 95%, as assessed by SDS-PAGE and HPLC analysis. High purity ensures robust performance and reliability in various immunoassays.
Q 3: How does clone M1709C9 compare to polyclonal antibodies in terms of specificity and reproducibility?
A: Clone M1709C9 offers higher specificity and reproducibility compared to polyclonal antibodies. As a monoclonal antibody, it targets a single epitope, reducing cross-reactivity and providing consistent results across different batches.

View the frequently asked questions answered by Creative Biolabs Support.